Wasylyk B, Wasylyk C, Matthes H, Wintzerith M, Chambon P
Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médicine, Strasbourg, France.
EMBO J. 1983;2(9):1605-11. doi: 10.1002/j.1460-2075.1983.tb01631.x.
Transcription for a hybrid SV40 promoter-beta globin coding sequence recombinant initiates from both early-early (EE) and late-early (LE) SV40 start sites (EES and LES) in the absence of DNA replication. The 72-bp repeat is essential to potentiate the elements of the two overlapping EE and LE promoters (EEP and LEP). Two current models, which can account for the EE to LE shift in RNA chain initiation during the SV40 replication cycle, are that LE transcription is linked to replication and occurs on newly replicated DNA molecules or that there are two promoter elements, a stronger EEP and a weaker LEP, T antigen repressing the EEP late in infection. Our results support the second model. A 5'-TATTTAT-3' to 5'-TATCGAT-3' mutation in the putative SV40 TATA box decreases transcription from EES, increases transcription from LES, and inhibits DNA replication. Therefore, this element acts as a classical TATA box for transcription, and yet is also important for DNA replication.
在没有DNA复制的情况下，杂交的SV40启动子-β珠蛋白编码序列重组体的转录起始于早期-早期（EE）和晚期-早期（LE）SV40起始位点（EES和LES）。72碱基对重复序列对于增强两个重叠的EE和LE启动子（EEP和LEP）的元件至关重要。目前有两种模型可以解释SV40复制周期中RNA链起始从EE到LE的转变，一种是LE转录与复制相关，发生在新复制的DNA分子上；另一种是有两个启动子元件，一个较强的EEP和一个较弱的LEP，T抗原在感染后期抑制EEP。我们的结果支持第二种模型。假定的SV40 TATA框中从5'-TATTTAT-3'到5'-TATCGAT-3'的突变降低了从EES的转录，增加了从LES的转录，并抑制了DNA复制。因此，该元件作为转录的经典TATA框，同时对DNA复制也很重要。
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