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酵母RNA聚合酶II在体外于靠近DNA模板潜在非B型结构的TATA序列处起始转录。

Yeast RNA polymerase II initiates transcription in vitro at TATA sequences proximal to potential non-B forms of the DNA template.

作者信息

Lescure B, Arcangioli B

出版信息

EMBO J. 1984 May;3(5):1067-73. doi: 10.1002/j.1460-2075.1984.tb01929.x.

DOI:10.1002/j.1460-2075.1984.tb01929.x
PMID:6376116
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557473/
Abstract

Pure yeast RNA polymerase II selectively initiates an abortive in vitro transcript within a TATA box of the yeast iso-1 cytochrome c gene promoter. Using a series of promoter deletions we show that a DNA sequence located upstream of the TATA box is needed for an efficient in vitro transcription. Supercoiling of the DNA template is an absolute requirement for the specific in vitro transcription. Examination of the DNA structure near several in vitro initiation sites shows that the common features observed are the presence of a TATA sequence in which RNA synthesis is initiated, and which is proximal to a potential non-B form of the DNA (a B to Z transition or a cruciform structure).

摘要

纯酵母RNA聚合酶II在酵母异-1细胞色素c基因启动子的TATA框内选择性地引发体外流产转录。通过一系列启动子缺失实验，我们发现TATA框上游的DNA序列对于高效的体外转录是必需的。DNA模板的超螺旋是特异性体外转录的绝对要求。对几个体外起始位点附近的DNA结构进行检查发现，观察到的共同特征是存在一个TATA序列，RNA合成在此处起始，并且该序列靠近DNA的潜在非B型结构（B到Z转变或十字形结构）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/2bc5143fa9e7/emboj00309-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/3ba3ba5d5ef0/emboj00309-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/e55feb7f093b/emboj00309-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/1a53632564f0/emboj00309-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/20c75f308e2e/emboj00309-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/2bc5143fa9e7/emboj00309-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/3ba3ba5d5ef0/emboj00309-0148-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/e55feb7f093b/emboj00309-0148-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/1a53632564f0/emboj00309-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/20c75f308e2e/emboj00309-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb3b/557473/2bc5143fa9e7/emboj00309-0151-a.jpg

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引用本文的文献

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本文引用的文献

1
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EMBO J. 1983;2(9):1605-11. doi: 10.1002/j.1460-2075.1983.tb01631.x.
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Multiple factors required for accurate initiation of transcription by purified RNA polymerase II.纯化的RNA聚合酶II准确起始转录需要多种因素。
J Biol Chem. 1980 Dec 25;255(24):11992-6.
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Localization of DNA sequences necessary for transcription of the rabbit beta-globin gene in vitro.
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High rotational mobility of DNA in animal cells and its modulation by histone acetylation.动物细胞中DNA的高旋转流动性及其通过组蛋白乙酰化的调节。
Mol Gen Genet. 1991 Dec;231(1):17-21. doi: 10.1007/BF00293816.
体外兔β-珠蛋白基因转录所需DNA序列的定位
Cell. 1981 Jul;25(1):215-26. doi: 10.1016/0092-8674(81)90246-4.
4
Negatively supercoiled plasmids contain left-handed Z-DNA segments as detected by specific antibody binding.通过特异性抗体结合检测发现，负超螺旋质粒含有左手Z-DNA片段。
Cell. 1982 Dec;31(2 Pt 1):309-18. doi: 10.1016/0092-8674(82)90124-6.
5
The nucleotide sequence of the HIS4 region of yeast.酵母HIS4区域的核苷酸序列。
Gene. 1982 Apr;18(1):47-59. doi: 10.1016/0378-1119(82)90055-5.
6
Efficient and selective initiation by yeast RNA polymerase B in a dinucleotide-primed reaction.酵母RNA聚合酶B在二核苷酸引发反应中的高效且选择性起始。
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Histone H2B genes of yeast encode two different proteins.酵母的组蛋白H2B基因编码两种不同的蛋白质。
Cell. 1980 Dec;22(3):799-805. doi: 10.1016/0092-8674(80)90556-5.
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