TATA框上游富含GC的重复基序是SV40早期启动子的重要元件。
The repeated GC-rich motifs upstream from the TATA box are important elements of the SV40 early promoter.
作者信息
Everett R D, Baty D, Chambon P
出版信息
Nucleic Acids Res. 1983 Apr 25;11(8):2447-64. doi: 10.1093/nar/11.8.2447.
Abstract
We have constructed deletion and point mutations within the Simian virus 40 (SV40) early promoter region which contains two tandemly repeated 21 bp sequences and a related 22 bp sequence (the "upstream" 21 bp repeat region). After transfection into permissive CV-1 cells and non-permissive mouse 3T3-4E cells, the effect of the mutations on early gene expression was studied by measuring T-antigen production, using indirect immunofluoresence. Our results demonstrate that the 21 bp repeat region, and in particular the six GC-rich motifs 5'-CCGCCC-3' which are repeated in this region constitute an important element of the SV40 early promoter. Surprisingly, we found that the requirement for the 21 bp repeat region for early gene expression was partially fulfilled even when it was in the inverted orientation.
摘要
我们在猴病毒40（SV40）早期启动子区域构建了缺失和点突变，该区域包含两个串联重复的21bp序列和一个相关的22bp序列（“上游”21bp重复区域）。转染到允许性CV-1细胞和非允许性小鼠3T3-4E细胞后，通过间接免疫荧光法测量T抗原的产生，研究了这些突变对早期基因表达的影响。我们的结果表明，21bp重复区域，特别是该区域中重复的六个富含GC的基序5'-CCGCCC-3'，构成了SV40早期启动子的一个重要元件。令人惊讶的是，我们发现即使21bp重复区域处于反向排列时，早期基因表达对其的需求也能部分得到满足。
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