Moison Ralf M W, Beijersbergen van Henegouwen Gerard M J
Department of Medicinal Photochemistry, Leiden/Amsterdam Center for Drug Research, University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Radiat Res. 2002 Apr;157(4):402-9. doi: 10.1667/0033-7587(2002)157[0402:tavcae]2.0.co;2.
Eicosapentaenoic acid protects against UV-radiation-induced immunosuppression and photocarcinogenesis, but it is also prone to oxidative degradation, which may reduce or abolish its beneficial effects. The protective effect of topically applied vitamin E, vitamin C, or both against UVB-radiation-induced lipid peroxidation in the presence of eicosapentaenoic acid was investigated using an ex vivo pig skin model. Changes in the bioavailability of both antioxidants induced by UV radiation were studied in different skin compartments. The UVB-radiation dose used (25 kJ/m2) was similar to that required to induce immunosuppression in BALB/c mice. Exposure of pig skin with an epidermal eicosapentaenoic acid content of 1.0 +/- 0.3 mol% to UVB radiation resulted in an 85% increase of epidermal lipid peroxidation (P < 0.005). Topical application of vitamin E or vitamin C 60 min prior to UVB irradiation resulted in a major increase in both antioxidants in the stratum corneum and viable epidermis (P < 0.05). Vitamin E and vitamin C completely protected against UVB-radiation-induced lipid peroxidation (P < 0.005), but compared to vitamin E, a 500-fold higher vitamin C dose was needed. UVB irradiation induced a vitamin E consumption of up to 100% in the stratum corneum and viable epidermis, and a vitamin C consumption of only 21% in the stratum corneum. Simultaneously applied vitamin E and vitamin C also completely protected against UVB-radiation-induced lipid peroxidation (P < 0.05), and lower antioxidant doses were needed compared to vitamin E or vitamin C alone. In the presence of vitamin C, epidermal vitamin E was more stable upon UVB irradiation (P < 0.05), suggesting interaction between vitamin E and vitamin C. In conclusion, topically applied vitamin E and/or vitamin C efficiently protect against UVB-radiation-induced lipid peroxidation in the presence of eicosapentaenoic acid. The beneficial biological effects of eicosapentaenoic acid may therefore be improved if vitamin E and/or vitamin C are present in sufficient amounts. The ex vivo pig skin model provides a useful tool for assessing short-term biochemical effects related to UVB radiation, without the use of living experimental animals.
二十碳五烯酸可预防紫外线辐射诱导的免疫抑制和光致癌作用，但它也易于发生氧化降解，这可能会降低或消除其有益作用。使用离体猪皮肤模型研究了局部应用维生素E、维生素C或两者对二十碳五烯酸存在下紫外线B辐射诱导的脂质过氧化的保护作用。研究了紫外线辐射在不同皮肤层中诱导的两种抗氧化剂生物利用度的变化。所使用的紫外线B辐射剂量（25 kJ/m2）与诱导BALB/c小鼠免疫抑制所需的剂量相似。将表皮二十碳五烯酸含量为1.0±0.3 mol%的猪皮肤暴露于紫外线B辐射下，导致表皮脂质过氧化增加85%（P<0.005）。在紫外线B照射前60分钟局部应用维生素E或维生素C，导致角质层和活表皮中两种抗氧化剂均显著增加（P<0.05）。维生素E和维生素C完全预防了紫外线B辐射诱导的脂质过氧化（P<0.005），但与维生素E相比，需要高500倍的维生素C剂量。紫外线B照射导致角质层和活表皮中维生素E消耗高达100%，而角质层中维生素C消耗仅为21%。同时应用维生素E和维生素C也完全预防了紫外线B辐射诱导的脂质过氧化（P<0.05），与单独使用维生素E或维生素C相比，所需的抗氧化剂剂量更低。在维生素C存在下，紫外线B照射时表皮维生素E更稳定（P<0.05），表明维生素E和维生素C之间存在相互作用。总之，在二十碳五烯酸存在下，局部应用维生素E和/或维生素C可有效预防紫外线B辐射诱导的脂质过氧化。因此，如果存在足够量的维生素E和/或维生素C，二十碳五烯酸的有益生物学效应可能会得到改善。离体猪皮肤模型为评估与紫外线B辐射相关的短期生化效应提供了一个有用的工具，而无需使用活体实验动物。
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