• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

腺病毒12S和13S EIa信使核糖核酸的个别产物在转录水平上刺激病毒EIIa和EIII的表达。

Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.

作者信息

Leff T, Elkaim R, Goding C R, Jalinot P, Sassone-Corsi P, Perricaudet M, Kédinger C, Chambon P

出版信息

Proc Natl Acad Sci U S A. 1984 Jul;81(14):4381-5. doi: 10.1073/pnas.81.14.4381.

DOI:10.1073/pnas.81.14.4381
PMID:6336330
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC345593/
Abstract

Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the level of specific transcription in nuclei isolated from transfected cells we directly demonstrate that the increased amount of EIIa RNA is due to stimulation of the rate of transcription.

摘要

将含有仅产生12S mRNA、仅产生13S mRNA或同时产生这两种mRNA的突变型或野生型腺病毒2型EIa基因的重组质粒，与含有病毒EIIa或EIII转录单位的质粒共转染到HeLa细胞中。13S和12S RNA的产物均增加了从EIIa和EIII启动子产生的RNA量。通过测量从转染细胞中分离出的细胞核中的特异性转录水平，我们直接证明EIIa RNA量的增加是由于转录速率的刺激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/7090422fff9a/pnas00615-0151-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/26459c2a1495/pnas00615-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/2c3fdb2a6610/pnas00615-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/bc7a0ddc9f5c/pnas00615-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/abb9ea6e4d85/pnas00615-0151-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/7090422fff9a/pnas00615-0151-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/26459c2a1495/pnas00615-0149-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/2c3fdb2a6610/pnas00615-0150-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/bc7a0ddc9f5c/pnas00615-0151-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/abb9ea6e4d85/pnas00615-0151-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f8bb/345593/7090422fff9a/pnas00615-0151-c.jpg

相似文献

1
Individual products of the adenovirus 12S and 13S EIa mRNAs stimulate viral EIIa and EIII expression at the transcriptional level.腺病毒12S和13S EIa信使核糖核酸的个别产物在转录水平上刺激病毒EIIa和EIII的表达。
Proc Natl Acad Sci U S A. 1984 Jul;81(14):4381-5. doi: 10.1073/pnas.81.14.4381.
2
Sequence-specific trans-activation of the adenovirus EIIa early promoter by the viral EIV transcription unit.病毒EIV转录单元对腺病毒EIIa早期启动子的序列特异性反式激活。
EMBO J. 1985 Jun;4(6):1523-8. doi: 10.1002/j.1460-2075.1985.tb03812.x.
3
EIa-mediated stimulation of the adenovirus EIII promoter involves an enhancer element within the nearby EIIa promoter.E1a介导的腺病毒EIII启动子刺激涉及附近EIIa启动子内的一个增强子元件。
J Virol. 1988 May;62(5):1762-7. doi: 10.1128/JVI.62.5.1762-1767.1988.
4
Sequence-specific activation of transcription by adenovirus EIa products is observed in HeLa cells but not in 293 cells.在HeLa细胞中观察到腺病毒E1a产物对转录的序列特异性激活，但在293细胞中未观察到。
Mol Cell Biol. 1986 Jan;6(1):201-8. doi: 10.1128/mcb.6.1.201-208.1986.
5
Splicing of adenovirus 2 early region 1A mRNAs is non-sequential.腺病毒2型早期区域1A信使核糖核酸的剪接是不连续的。
J Mol Biol. 1983 Apr 15;165(3):475-95. doi: 10.1016/s0022-2836(83)80214-9.
6
Adenovirus EIIA early promoter: transcriptional control elements and induction by the viral pre-early EIA gene, which appears to be sequence independent.腺病毒EIIA早期启动子：转录控制元件以及由病毒早期前EIA基因的诱导作用，这种诱导作用似乎与序列无关。
Proc Natl Acad Sci U S A. 1985 Apr;82(8):2230-4. doi: 10.1073/pnas.82.8.2230.
7
Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products.腺病毒5型EIII启动子的转录分析：EIa基因产物刺激不存在序列特异性。
Nucleic Acids Res. 1985 Feb 25;13(4):1209-21. doi: 10.1093/nar/13.4.1209.
8
Modulation of alternative splicing of adenoviral E1A transcripts: factors involved in the early-to-late transition.腺病毒E1A转录本可变剪接的调控：参与早期到晚期转变的因子
Genes Dev. 1991 Oct;5(10):1847-58. doi: 10.1101/gad.5.10.1847.
9
Regulation of transcription of the adenovirus EII promoter by EIa gene products: absence of sequence specificity.腺病毒EII启动子转录受EIa基因产物的调控：缺乏序列特异性。
Mol Cell Biol. 1984 Oct;4(10):1970-7. doi: 10.1128/mcb.4.10.1970-1977.1984.
10
Selective inhibition of adenovirus type 2 early region II and III transcription by an anisomycin block of protein synthesis.通过茴香霉素阻断蛋白质合成对2型腺病毒早期区域II和III转录的选择性抑制。
Mol Cell Biol. 1982 Jul;2(7):789-99. doi: 10.1128/mcb.2.7.789-799.1982.

引用本文的文献

1
Adenovirus protein VII condenses DNA, represses transcription, and associates with transcriptional activator E1A.腺病毒蛋白VII使DNA浓缩，抑制转录，并与转录激活因子E1A结合。
J Virol. 2004 Jun;78(12):6459-68. doi: 10.1128/JVI.78.12.6459-6468.2004.
2
Transcriptional coactivator cAMP response element binding protein mediates induction of the human proliferating cell nuclear antigen promoter by the adenovirus E1A oncoprotein.转录共激活因子环磷酸腺苷反应元件结合蛋白介导腺病毒E1A癌蛋白对人增殖细胞核抗原启动子的诱导作用。
Proc Natl Acad Sci U S A. 1997 Apr 29;94(9):4481-6. doi: 10.1073/pnas.94.9.4481.
3
mRNA export correlates with activation of transcription in human subgroup C adenovirus-infected cells.

本文引用的文献

1
The E1b promoter of Ad12 in mouse L tk- cells is activated by adenovirus region E1a.腺病毒12型（Ad12）在小鼠L tk-细胞中的E1b启动子被腺病毒E1a区域激活。
EMBO J. 1983;2(1):73-6. doi: 10.1002/j.1460-2075.1983.tb01383.x.
2
The ovalbumin gene family: hormonal control of X and Y gene transcription and mRNA accumulation.卵清蛋白基因家族：X基因和Y基因转录及mRNA积累的激素调控
Cell. 1981 Feb;23(2):561-71. doi: 10.1016/0092-8674(81)90152-5.
3
Adenovirus early gene products may control viral mRNA accumulation and translation in vivo.腺病毒早期基因产物可能在体内控制病毒mRNA的积累和翻译。
mRNA输出与人类C亚组腺病毒感染细胞中的转录激活相关。
J Virol. 1996 Jun;70(6):4071-80. doi: 10.1128/JVI.70.6.4071-4080.1996.
4
Transcriptional activation by the adenovirus larger E1a product is mediated by members of the cellular transcription factor ATF family which can directly associate with E1a.腺病毒较大的E1a产物的转录激活是由细胞转录因子ATF家族的成员介导的，这些成员可以直接与E1a结合。
Mol Cell Biol. 1993 Jan;13(1):561-70. doi: 10.1128/mcb.13.1.561-570.1993.
5
Down-regulation of HLA antigens by the adenovirus type 2 E3/19K protein in a T-lymphoma cell line.2型腺病毒E3/19K蛋白对T淋巴瘤细胞系中HLA抗原的下调作用
J Virol. 1994 Mar;68(3):1442-8. doi: 10.1128/JVI.68.3.1442-1448.1994.
6
Conserved cysteine residues within the E3/19K protein of adenovirus type 2 are essential for binding to major histocompatibility complex antigens.2型腺病毒E3/19K蛋白内保守的半胱氨酸残基对于与主要组织相容性复合体抗原的结合至关重要。
J Virol. 1994 Sep;68(9):5423-32. doi: 10.1128/JVI.68.9.5423-5432.1994.
7
Complementary functions of E1a conserved region 1 cooperate with conserved region 3 to activate adenovirus serotype 5 early promoters.E1a保守区域1的互补功能与保守区域3协同作用以激活5型腺病毒早期启动子。
J Virol. 1994 Aug;68(8):4910-20. doi: 10.1128/JVI.68.8.4910-4920.1994.
8
Modulation of transcriptional activation of the proliferating cell nuclear antigen promoter by the adenovirus E1A 243-residue oncoprotein depends on proximal activators.腺病毒E1A 243个氨基酸残基的癌蛋白对增殖细胞核抗原启动子转录激活的调节取决于近端激活因子。
Mol Cell Biol. 1994 Jan;14(1):543-53. doi: 10.1128/mcb.14.1.543-553.1994.
9
Suppression of mutations in two Saccharomyces cerevisiae genes by the adenovirus E1A protein.腺病毒E1A蛋白对酿酒酵母两个基因中突变的抑制作用。
Mol Cell Biol. 1995 Jun;15(6):3227-37. doi: 10.1128/MCB.15.6.3227.
10
Synthesis in Escherichia coli of human adenovirus type 12 transforming proteins encoded by early region 1A 13S mRNA and 12S mRNA.人12型腺病毒早期区域1A 13S信使核糖核酸和12S信使核糖核酸编码的转化蛋白在大肠杆菌中的合成。
Proc Natl Acad Sci U S A. 1984 Oct;81(20):6300-4. doi: 10.1073/pnas.81.20.6300.
Cell. 1981 Feb;23(2):485-96. doi: 10.1016/0092-8674(81)90144-6.
4
Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.伴清蛋白和卵清蛋白基因转录的特异性体外起始及与腺病毒2型早期和晚期基因的比较。
Nature. 1980 Jun 5;285(5764):367-73. doi: 10.1038/285367a0.
5
Mechanism of activation of early viral transcription by the adenovirus E1A gene product.腺病毒E1A基因产物激活早期病毒转录的机制。
Cell. 1981 Oct;26(2 Pt 2):213-20. doi: 10.1016/0092-8674(81)90304-4.
6
Transformation-defective mutant of adenovirus type 5 containing a single altered E1a mRNA species.含有单一改变的E1a mRNA种类的5型腺病毒转化缺陷型突变体。
J Virol. 1981 Dec;40(3):657-64. doi: 10.1128/JVI.40.3.657-664.1981.
7
Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site.通过在mRNA剪接位点进行位点特异性诱变来解析重叠病毒基因的功能。
Nature. 1982 Feb 4;295(5848):380-4. doi: 10.1038/295380a0.
8
Specific in vitro initiation of transcription on the adenovirus type 2 early and late EII transcription units.在腺病毒2型早期和晚期EII转录单元上进行特定的体外转录起始。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7383-7. doi: 10.1073/pnas.78.12.7383.
9
Expression of early adenovirus genes requires a viral encoded acidic polypeptide.早期腺病毒基因的表达需要一种病毒编码的酸性多肽。
Proc Natl Acad Sci U S A. 1981 Oct;78(10):6121-5. doi: 10.1073/pnas.78.10.6121.
10
Specific in vitro transcription of conalbumin gene is drastically decreased by single-point mutation in T-A-T-A box homology sequence.伴清蛋白基因的特异性体外转录因T-A-T-A盒同源序列中的单点突变而急剧减少。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7024-8. doi: 10.1073/pnas.77.12.7024.