Aleström P, Akusjärvi G, Perricaudet M, Mathews M B, Klessig D F, Pettersson U
Cell. 1980 Mar;19(3):671-81. doi: 10.1016/s0092-8674(80)80044-4.
A 606 base pair segment of adenovirus type 2 DNA which includes the gene for polypeptide IX and its flanking regions has been sequenced. Comparison with the sequence of a cDNA copy of the 9S mRNA for polypeptide IX and direct analysis of its capped oligonucleotide show that the gene and its mRNA are colinear. The 9S mRNA has a length of 485 nucleotides, excluding the poly(A) tract and the m7G of the cap, and contains one single open translational reading frame which is sufficiently long to encode polypeptide IX. This reading frame contains three AUG codons, the first of which is used for initiation of protein synthesis. The mRNA has a 5' noncoding region of 24 nucleotides, excluding the m5G of the cap, and encodes a polypeptide which is 139 amino acids long and unusually rich in serine and alanine. The sequence TATATAA is found 25 nucleotides upstream from the cap site. The polyadenylation site has been identified but cannot be located unambiguously because of the presence of two A residues in the DNA sequence at this position. The 3' noncoding region is 41--43 nucleotides long and contains the hexanucleotide sequence AAUAAA. The mRNA for polypeptide IVa2 is encoded adjacent to that for polypeptide IX and is transcribed in the opposite direction. Sequence analysis revealed an overlap of 11--16 nucleotides, depending on the precise location of the polyadenylation sites, between 3' ends of these mRNAs.
已经对包含腺病毒2型DNA的一段606个碱基对的片段进行了测序，该片段包括多肽IX基因及其侧翼区域。将其与多肽IX的9S mRNA的cDNA拷贝序列进行比较，并对其加帽寡核苷酸进行直接分析，结果表明该基因与其mRNA是共线性的。9S mRNA长度为485个核苷酸，不包括聚腺苷酸尾和帽的m7G，并且包含一个单一的开放翻译阅读框，其长度足以编码多肽IX。这个阅读框包含三个AUG密码子，其中第一个用于蛋白质合成的起始。该mRNA具有24个核苷酸的5'非编码区，不包括帽的m5G，并且编码一个长度为139个氨基酸的多肽，该多肽富含丝氨酸和丙氨酸。在帽位点上游25个核苷酸处发现序列TATATAA。已经确定了聚腺苷酸化位点，但由于该位置的DNA序列中存在两个A残基，因此无法明确其位置。3'非编码区长41 - 43个核苷酸，并且包含六核苷酸序列AAUAAA。多肽IVa2的mRNA与多肽IX的mRNA相邻编码，并且转录方向相反。序列分析显示，根据聚腺苷酸化位点的精确位置，这些mRNA的3'末端之间存在11 - 16个核苷酸的重叠。
