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一个与HLA - DR抗原β链对应的cDNA克隆的分离与鉴定。

Isolation and identification of a cDNA clone corresponding to an HLA-DR antigen beta chain.

作者信息

Wiman K, Larhammar D, Claesson L, Gustafsson K, Schenning L, Bill P, Böhme J, Denaro M, Dobberstein B, Hammerling U, Kvist S, Servenius B, Sundelin J, Peterson P A, Rask L

出版信息

Proc Natl Acad Sci U S A. 1982 Mar;79(6):1703-7. doi: 10.1073/pnas.79.6.1703.

DOI:10.1073/pnas.79.6.1703
PMID:6952222
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346048/
Abstract

The HLA-D locus in the major histocompatibility complex controls the expression of the genetically polymorphic HLA-DR antigens. mRNA coding for the beta chains of these antigens was partially purified from the human lymphoblastoid cell line Raji. The mRNA was copied into double-stranded cDNA and cloned in Escherichia coli. One clone, pDR-beta-1, obtained by hybrid selection, carries a 1070-base-pair insert comprising all of the coding region except the signal sequence and a substantial portion of the untranslated region. To identify pDR-beta-1, highly purified HLA-DR antigen beta chains derived from Raji cells were subjected to NH2-terminal amino acid sequence determination. This sequence displayed extensive homology with that deduced from the nucleotide sequence at the 5' end of the pDR-beta-1 coding region. Taken together, the amino acid and nucleotide sequences strongly argue in favor of Raji cells containing at least two beta-chain loci.

摘要

主要组织相容性复合体中的HLA - D位点控制着遗传多态性HLA - DR抗原的表达。编码这些抗原β链的mRNA是从人淋巴母细胞系Raji中部分纯化得到的。该mRNA被转录成双链cDNA并克隆到大肠杆菌中。通过杂交筛选获得的一个克隆pDR - β - 1，携带一个1070个碱基对的插入片段，该片段包含除信号序列外的所有编码区以及大部分非翻译区。为了鉴定pDR - β - 1，对来自Raji细胞的高度纯化的HLA - DR抗原β链进行了氨基末端氨基酸序列测定。该序列与从pDR - β - 1编码区5'端核苷酸序列推导出来的序列显示出广泛的同源性。综合来看，氨基酸和核苷酸序列有力地表明Raji细胞至少含有两个β链基因座。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d73/346048/291f78255a8d/pnas00445-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d73/346048/291f78255a8d/pnas00445-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6d73/346048/291f78255a8d/pnas00445-0030-a.jpg

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本文引用的文献

1
Structural polymorphism of human DR antigens.人类 DR 抗原的结构多态性。
Nature. 1979 May 31;279(5712):436-7. doi: 10.1038/279436a0.
2
The isolation of plasmids containing DNA complementary to messenger RNA for variant surface glycoproteins of Trypanosoma brucei.含有与布氏锥虫可变表面糖蛋白信使核糖核酸互补的DNA的质粒的分离。
Gene. 1980 Mar;8(4):391-417. doi: 10.1016/0378-1119(80)90043-8.
3
Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.主要组织相容性抗原：人类（HLA - A、- B、- C）和小鼠（H - 2K、H - 2D）I类分子。
根据核苷酸序列预测的一种HLA - DR抗原样β链的完整氨基酸序列：与免疫球蛋白以及HLA - A、- B和 - C抗原的相似性。
Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687-91. doi: 10.1073/pnas.79.12.3687.
4
Translation and assembly of HLA-DR antigens in Xenopus oocytes injected with mRNA from a human B-cell line.在注射了来自人B细胞系mRNA的非洲爪蟾卵母细胞中HLA-DR抗原的翻译与组装。
EMBO J. 1982;1(5):649-54. doi: 10.1002/j.1460-2075.1982.tb01222.x.
5
Isolation of distinct cDNA clones encoding HLA-DR beta chains by use of an expression assay.通过表达分析分离编码HLA - DRβ链的不同cDNA克隆。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7465-9. doi: 10.1073/pnas.79.23.7465.
6
cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain.人B细胞同种异体抗原DC1重链的cDNA克隆：与HLA - DR重链有高度序列同源性。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6337-41. doi: 10.1073/pnas.79.20.6337.
7
A novel HLA-D/DR-like antigen specific for human B lymphoid cells. Biochemical evidence for similarity to but nonidentity with known HLA-D/DR antigens.一种对人B淋巴细胞特异的新型HLA - D/DR样抗原。与已知HLA - D/DR抗原相似但不同一的生化证据。
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9
Beta 2-microglobulin induces intracellular transport of human class I transplantation antigen heavy chains in Xenopus laevis oocytes.β2-微球蛋白诱导非洲爪蟾卵母细胞中人类I类移植抗原重链的细胞内运输。
J Cell Biol. 1984 Jul;99(1 Pt 1):226-32. doi: 10.1083/jcb.99.1.226.
10
Mapping of the genes encoding the HLA-DR alpha chain and the HLA-related antigens to a chromosome 6 deletion by using genomic blotting.利用基因组印迹法将编码HLA - DRα链和HLA相关抗原的基因定位到6号染色体缺失区域。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2300-4. doi: 10.1073/pnas.80.8.2300.
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4
Structural polymorphism of the beta chain of human HLA-DR antigens.人类HLA - DR抗原β链的结构多态性
Mol Immunol. 1980 Dec;17(12):1443-8. doi: 10.1016/0161-5890(80)90169-8.
5
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9
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J Bacteriol. 1980 Aug;143(2):926-33. doi: 10.1128/jb.143.2.926-933.1980.
10
Biochemical comparison of HLA-DR molecules derived from autologous human T and B lymphoblasts.源自自体人T淋巴细胞和B淋巴细胞母细胞的HLA-DR分子的生化比较。
Eur J Immunol. 1980 Dec;10(12):908-14. doi: 10.1002/eji.1830101204.