Korman A J, Auffray C, Schamboeck A, Strominger J L
Proc Natl Acad Sci U S A. 1982 Oct;79(19):6013-7. doi: 10.1073/pnas.79.19.6013.
The amino acid sequence of the heavy chain of HLA-DR antigens has been elucidated from the analysis of a genomic clone coding for this protein. A 3.2-kilobase EcoRI fragment includes four exons containing 227 amino acids out of 229 in the mature HLA-DR heavy chain. One exon (alpha 2) encodes a domain of 94 amino acids with strong sequence homology both to Ig constant region domains and to Ig-like domains in HLA-B7, beta 2-microglobulin, and the HLA-DR light chain. These results support a structure for the HLA-DR antigen heterodimer consisting of four extracellular domains, two of which are Ig-like [one in the heavy chain (alpha 2) and one in the light chain (beta 2)]. The third is the amino-terminal polymorphic domain in the light chain (beta 1), and the fourth is an invariant domain in the heavy chain (alpha 1).
通过对编码该蛋白的基因组克隆进行分析，已阐明了HLA - DR抗原重链的氨基酸序列。一个3.2千碱基的EcoRI片段包含四个外显子，这些外显子编码成熟HLA - DR重链中229个氨基酸中的227个。一个外显子（α2）编码一个含有94个氨基酸的结构域，该结构域与Ig恒定区结构域以及HLA - B7、β2 - 微球蛋白和HLA - DR轻链中的Ig样结构域具有很强的序列同源性。这些结果支持了HLA - DR抗原异二聚体的结构，该异二聚体由四个细胞外结构域组成，其中两个是Ig样结构域[一个在重链（α2）中，一个在轻链（β2）中]。第三个是轻链（β1）中的氨基末端多态性结构域，第四个是重链（α1）中的恒定结构域。
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