Schröder G, Klipp W, Hillebrand A, Ehring R, Koncz C, Schröder J
Max-Planck-Institut für Züchtungsforschung, Lehrstuhl für Genetik, Universität Bielefeld, FRG.
EMBO J. 1983;2(3):403-9. doi: 10.1002/j.1460-2075.1983.tb01437.x.
The T-region of Ti-plasmids expresses four proteins (mol. wts. 74,000, 49,000, 28,000 and 27,000) in Escherichia coli minicells. Promoter activities are determined by sequences within the T-region, and the protein-coding regions map in that part of the T-region which is highly conserved in octopine and nopaline plasmids and which is responsible for shoot and root inhibition when expressed in plant cells. Three of the regions expressed in bacteria correlate with three regions which are transcribed in transformed plant cells; the fourth protein-coding region has no corresponding transcript in plants. At least three of the proteins synthesized in E. coli minicells are also expressed in cell-free systems prepared from E. coli and from Agrobacterium tumefaciens; the fourth protein (mol. wt. 49,000) is poorly expressed in both cell-free extracts. The possibility is discussed that the same genes are expressed in Agrobacteria and in transformed plant cells and that in both cases the gene products mediate growth regulatory effects to non-transformed plant cells.
Ti 质粒的 T 区域在大肠杆菌小细胞中表达四种蛋白质（分子量分别为 74,000、49,000、28,000 和 27,000）。启动子活性由 T 区域内的序列决定，蛋白质编码区位于 T 区域的一部分，该部分在章鱼碱和胭脂碱质粒中高度保守，并且在植物细胞中表达时负责抑制芽和根的生长。在细菌中表达的三个区域与在转化植物细胞中转录的三个区域相关；第四个蛋白质编码区在植物中没有相应的转录本。在大肠杆菌小细胞中合成的蛋白质中，至少有三种也在由大肠杆菌和根癌土壤杆菌制备的无细胞系统中表达；第四种蛋白质（分子量 49,000）在两种无细胞提取物中的表达都很差。文中讨论了一种可能性，即相同的基因在土壤杆菌和转化植物细胞中表达，并且在这两种情况下，基因产物都对未转化的植物细胞介导生长调节作用。
