Wiborg O, Hyldig-Nielsen J J, Jensen E O, Paludan K, Marcker K A
Department of Molecular Biology and Plant Physiology, University of Aarhus, Denmark.
EMBO J. 1983;2(3):449-52. doi: 10.1002/j.1460-2075.1983.tb01443.x.
A clone containing an unusual leghemoglobin (Lb) gene was isolated from a soybean DNA library present in Charon 4A phage. DNA sequence analysis revealed that the isolated Lb gene has three intervening sequences (IVS-1, IVS-2 and IVS-3) located in the same positions as those found in other Lb genes. Due to a large increase of IVS-2 and IVS-3, the isolated Lb gene is about twice the size of a normal Lb gene. The coding sequence derived from the DNA sequence corresponds to no known soybean Lb and attempts to find a corresponding mRNA failed. In addition, the 5'-flanking sequence of the Lb gene is mutated in two regions which seem to be important for transcription. It is, therefore, tentatively suggested that the isolated Lb gene is non-functional, and consequently is an Lb pseudogene.
从存在于Charon 4A噬菌体中的大豆DNA文库中分离出一个含有异常豆血红蛋白(Lb)基因的克隆。DNA序列分析表明,分离出的Lb基因有三个间隔序列(IVS - 1、IVS - 2和IVS - 3),其位置与其他Lb基因中的相同。由于IVS - 2和IVS - 3大幅增加,分离出的Lb基因大小约为正常Lb基因的两倍。从DNA序列推导的编码序列与任何已知的大豆Lb均不对应,并且寻找相应mRNA的尝试也失败了。此外,Lb基因的5'侧翼序列在两个似乎对转录很重要的区域发生了突变。因此,初步推测分离出的Lb基因无功能,因而它是一个Lb假基因。