Anderson C R, Jensen E O, LLewellyn D J, Dennis E S, Peacock W J
Commonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, Australia.
Proc Natl Acad Sci U S A. 1996 Jun 11;93(12):5682-7. doi: 10.1073/pnas.93.12.5682.
We have isolated a new hemoglobin gene from soybean. It is expressed in cotyledons, stems of seedlings, roots, young leaves, and in some cells in the nodules that are associated with the nitrogen-fixing Bradyrhizobium symbiont. This contrasts with the expression of the leghemoglobins, which are active only in the infected cells of the nodules. The deduced protein sequence of the new gene shows only 58% similarity to one of the soybean leghemoglobins, but 85-87% similarity to hemoglobins from the nonlegumes Parasponia, Casuarina, and barley. The pattern of expression and the gene sequence indicate that this new gene is a nonsymbiotic legume hemoglobin. The finding of this gene in legumes and similar genes in other species strengthens our previous suggestion that genomes of all plants contain hemoglobin genes. The specialized leghemoglobin gene family may have arisen from a preexisting nonsymbiotic hemoglobin by gene duplication.
我们从大豆中分离出了一个新的血红蛋白基因。它在子叶、幼苗茎、根、幼叶以及与固氮慢生根瘤菌共生的根瘤中的一些细胞中表达。这与豆血红蛋白的表达情况形成对比，豆血红蛋白仅在根瘤的受感染细胞中具有活性。新基因推导的蛋白质序列与一种大豆豆血红蛋白的相似度仅为58%，但与非豆科植物胡颓子、木麻黄和大麦的血红蛋白相似度为85 - 87%。表达模式和基因序列表明这个新基因是一种非共生豆科血红蛋白。在豆科植物中发现该基因以及在其他物种中发现类似基因，强化了我们之前的推测，即所有植物的基因组都包含血红蛋白基因。专门的豆血红蛋白基因家族可能是由一个先前存在的非共生血红蛋白通过基因复制产生的。
Zotero 插件