Grosveld G C, Rosenthal A, Flavell R A
Nucleic Acids Res. 1982 Aug 25;10(16):4951-71. doi: 10.1093/nar/10.16.4951.
We have performed a detailed analysis of DNA sequences in the -80 region of the rabbit beta-globin gene that are required for transcription. A variety of rabbit beta-globin gene templates deleted at various sites in the -80 region were linked to an SV40-plasmid recombinant and introduced into HeLa cells; under these conditions the rabbit beta-globin gene is expressed from its own promotor. The results show that the conserved GGCCAATCT sequence is required for efficient transcription in vivo and further identify another sequence in the region from about -81 to -96 which is also required for transcription in vivo.
我们对兔β-珠蛋白基因-80区域中参与转录所需的DNA序列进行了详细分析。在-80区域不同位点缺失的多种兔β-珠蛋白基因模板与SV40-质粒重组体相连，并导入HeLa细胞；在这些条件下，兔β-珠蛋白基因通过自身启动子表达。结果表明，保守的GGCCAATCT序列是体内高效转录所必需的，并进一步确定了该区域中大约-81至-96处的另一个序列，该序列也是体内转录所必需的。
