Sparrow Rosemary L, Cauchi Jennifer A, Ramadi Lanny T, Waugh Caryll M, Kirkland Mark A
Development Unit, Australian Red Cross Blood Service, Melbourne, Victoria, Australia.
Transfusion. 2002 Feb;42(2):210-5. doi: 10.1046/j.1537-2995.2002.00028.x.
The aim of this study was to determine the influence of mode of birth and umbilical cord blood (CB) collection before (in utero) or after delivery of the placenta (ex utero) on total number of WBCs and CD34+ cells in CB units.
Consecutively donated, banked CB units were assessed for net volume, WBC concentration, total number of WBCs, proportion of CD34+ cells, and total number of CD34+ cells. These parameters were then correlated with the mode of birth and the mode of CB collection relative to the delivery of the placenta.
A significantly higher CB volume was seen following cesarean section (n = 61) than following vaginal delivery (n = 157; median volume, 76 vs. 63 mL, respectively; p < 0.0001). In contrast, CB from vaginal delivery had a significantly higher WBC concentration compared with CB from cesarean section (medians, 17.1 x 10(9) and 13.6 x 10(9) WBCs/L, respectively; p < 0.0001). The mode of birth did not influence the proportion of CD34+ cells. A correlation was demonstrated between the total number of CD34+ cells and the total number of WBCs. As a consequence of the opposing effects on volume and WBC counts by cesarean section and vaginal delivery, there were no significant differences in the total number of WBCs or CD34+ cells for the CB units with mode of delivery in this study. No significant differences were found in CB with mode of CB collection (in utero [n = 58] or ex utero [n = 99]) following vaginal delivery.
The mode of birth influences the CB WBC concentration and volume collected and should be taken into consideration for establishing any acceptance limits for CB units to be banked. There were no differences in CB with in utero or ex utero collections.
本研究的目的是确定分娩方式以及胎盘娩出前（宫内）或娩出后（宫外）采集脐带血（CB）对CB单位中白细胞（WBC）总数和CD34+细胞数量的影响。
对连续捐赠并储存的CB单位进行净体积、WBC浓度、WBC总数、CD34+细胞比例以及CD34+细胞总数的评估。然后将这些参数与分娩方式以及相对于胎盘娩出的CB采集方式相关联。
剖宫产（n = 61）后采集的CB体积明显高于阴道分娩（n = 157）后采集的CB体积（中位数体积分别为76 mL和63 mL；p < 0.0001）。相比之下，阴道分娩采集的CB的WBC浓度明显高于剖宫产采集的CB（中位数分别为17.1×10⁹和13.6×10⁹个WBC/L；p < 0.0001）。分娩方式不影响CD34+细胞的比例。CD34+细胞总数与WBC总数之间存在相关性。由于剖宫产和阴道分娩对体积和WBC计数的相反影响，本研究中不同分娩方式的CB单位的WBC总数或CD34+细胞总数没有显著差异。阴道分娩后，CB采集方式（宫内[n = 58]或宫外[n = 99]）之间未发现显著差异。
分娩方式会影响采集的CB的WBC浓度和体积，在确定任何CB单位储存的接受限度时应予以考虑。宫内或宫外采集的CB之间没有差异。
