Masuda Kenichi, Sakaguchi Masahiro, Saito Saburo, Deboer Douglas J, Yamashita Kohei, Hasegawa Atsuhiko, Ohno Koichi, Tsujimoto Hajime
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
Vet Dermatol. 2002 Feb;13(1):53-59. doi: 10.1046/j.0959-4493.2001.00277.x.
Three dogs were examined because of episodes of recurrent pruritic dermatitis in the spring, the season of Japanese cedar (Cryptomeria japonica, CJ) pollination in Japan. The dogs were shown to be sensitive to CJ pollen allergen using intradermal testing and antigen-specific IgE measurement. Fluorometric enzyme-linked immunosorbant assay (ELISA) showed increased concentrations of IgE specific to Cry j 1 and a negative result for Cry j 2 in the three dogs. The concentrations of IgE specific to Cry j 1 during the season of CJ pollination were higher than the concentrations found during the off-season in all the dogs, and the variation in the concentrations correlated with the variation in clinical signs. Peripheral blood mononuclear cells showed apparent proliferative responses to crude CJ pollen antigen and Cry j 1 during CJ pollination season. These findings indicated that Cry j 1 was the major allergen recognized by IgE and lymphocytes and resulted in the development of type I hypersensitivity to CJ pollen allergen in these atopic dogs.
三只狗因在春季（日本日本柳杉（Cryptomeria japonica，CJ）授粉季节）反复出现瘙痒性皮炎而接受检查。通过皮内试验和抗原特异性IgE测量，显示这些狗对CJ花粉过敏原敏感。荧光酶联免疫吸附测定（ELISA）显示，这三只狗中针对Cry j 1的IgE浓度升高，而针对Cry j 2的结果为阴性。在CJ授粉季节，所有狗中针对Cry j 1的IgE浓度均高于非授粉季节的浓度，且浓度变化与临床症状变化相关。在CJ授粉季节，外周血单个核细胞对粗制CJ花粉抗原和Cry j 1表现出明显的增殖反应。这些发现表明，Cry j 1是被IgE和淋巴细胞识别的主要过敏原，并导致这些特应性狗对CJ花粉过敏原产生I型超敏反应。
