Price Mitchell D, Merte Janna, Nichols Ruthann, Koladich Peter M, Tobe Stephen S, Bendena William G
Department of Biology, Queen's University, K7L 3N6, Kingston, Ontario, Canada.
Peptides. 2002 Apr;23(4):787-94. doi: 10.1016/s0196-9781(01)00649-0.
We identified a Drosophila melanogaster gene encoding a peptide that dramatically decreases spontaneous muscle contractions and, correspondingly, named the peptide flatline (FLT). This gene consisted of 4 exons and was cytologically localized to 32D2-3. Processing of a predicted 122 amino acid precursor would release pEVRYRQCYFNPISCF that differs from Manduca sexta allatostatin (Mas-AST) by one amino acid, Y4-->F4. FLT does not act as an allatostatin. In situ tissue hybridization further suggests FLT is a novel brain-gut peptide and specifically, the measured activity indicates that it is a potent myotropin. Despite its profound myotropic effect, pupae injected with FLT eclosed.
我们鉴定出一个果蝇基因，其编码的一种肽可显著减少肌肉的自发收缩，相应地，我们将该肽命名为“静止肽”（FLT）。该基因由4个外显子组成，细胞学定位在32D2-3。对预测的122个氨基酸前体进行加工后会释放出pEVRYRQCYFNPISCF，它与烟草天蛾咽侧体抑制素（Mas-AST）仅在一个氨基酸上存在差异，即Y4→F4。FLT并不作为一种咽侧体抑制素发挥作用。原位组织杂交进一步表明FLT是一种新型的脑肠肽，具体而言，所测得的活性表明它是一种有效的促肌动素。尽管其具有显著的促肌动效应，但注射了FLT的蛹仍能羽化。
