Karetsou Zoe, Kretsovali Adroniki, Murphy Carol, Tsolas Orestes, Papamarcaki Thomais
Laboratory of Biological Chemistry, University of Ioannina Medical School, 45110 Ioannina, Greece.
EMBO Rep. 2002 Apr;3(4):361-6. doi: 10.1093/embo-reports/kvf071. Epub 2002 Mar 15.
Prothymosin alpha (ProTalpha) is a histone H1-binding protein localized in sites of active transcription in the nucleus. We report here that ProTalpha physically interacts with the CREB-binding protein (CBP), which is a versatile transcription co-activator. Confocal laser scanning microscopy reveals that ProTalpha partially colocalizes with CBP in discrete subnuclear domains. Using transient transfections, we show that ProTalpha synergizes with CBP and stimulates AP1- and NF-kappaB-dependent transcription. Furthermore, overexpression of ProTalpha enhances the transactivation potential of CBP. These findings reveal a new function for ProTalpha in transcription activation, probably through CBP-mediated recruitment to different promoters.
前胸腺素α（ProTα）是一种与组蛋白H1结合的蛋白质，定位于细胞核中活跃转录的位点。我们在此报告，ProTα与CREB结合蛋白（CBP）发生物理相互作用，CBP是一种多功能转录共激活因子。共聚焦激光扫描显微镜显示，ProTα与CBP在离散的核内亚结构域部分共定位。通过瞬时转染，我们发现ProTα与CBP协同作用并刺激AP1和NF-κB依赖性转录。此外，ProTα的过表达增强了CBP的反式激活潜能。这些发现揭示了ProTα在转录激活中的新功能，可能是通过CBP介导招募到不同的启动子。
