前胸腺素α是哺乳动物细胞中的一种染色质重塑蛋白。
Prothymosin alpha is a chromatin-remodelling protein in mammalian cells.
作者信息
Gomez-Marquez J, Rodríguez P
机构信息
Departamento de Bioqu approximately ímica y Biolog approximately ía Molecular, Facultad de Biolog approximately ía, Universidad de Santiago de Compostela, 15706 Santiago de Compostela, Galicia, Spain.
出版信息
Biochem J. 1998 Jul 1;333 ( Pt 1)(Pt 1):1-3. doi: 10.1042/bj3330001.
Abstract
Prothymosin alpha (ProTalpha) is an abundant mammalian acidic nuclear protein whose expression is related to cell proliferation. Here we report that in HL-60 cells overexpressing ProTalpha, the accessibility of micrococcal nuclease to chromatin is strongly increased. In the DNA ladder generated by the nuclease activity, the sizes of the mononucleosome (146 bp, the DNA fragment that is bound to the histone octamer) and its multimers correspond to nucleosomes lacking histone H1. The percentage of histone-H1-depleted chromatin (active chromatin) is also higher in the cells overexpressing ProTalpha. On the basis of these and previous findings, we propose a biological role for ProTalpha in the remodelling of chromatin fibres through its interaction with histone H1.
摘要
前胸腺素α（ProTα）是一种丰富的哺乳动物酸性核蛋白，其表达与细胞增殖相关。在此我们报告，在过表达ProTα的HL-60细胞中，微球菌核酸酶对染色质的可及性显著增加。在由核酸酶活性产生的DNA梯状条带中，单核小体（146 bp，与组蛋白八聚体结合的DNA片段）及其多聚体的大小对应于缺乏组蛋白H1的核小体。在过表达ProTα的细胞中，组蛋白H1缺失的染色质（活性染色质）的百分比也更高。基于这些及先前的发现，我们提出ProTα通过与组蛋白H1相互作用在染色质纤维重塑中发挥生物学作用。
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本文引用的文献
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Overexpression of prothymosin alpha accelerates proliferation and retards differentiation in HL-60 cells.原胸腺素α的过表达加速HL-60细胞的增殖并延缓其分化。
Biochem J. 1998 May 1;331 ( Pt 3)(Pt 3):753-61. doi: 10.1042/bj3310753.
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Chromatin remodeling machines: similar motors, ulterior motives.染色质重塑机器：相似的动力，隐秘的动机。
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Turnover of the acyl phosphates of human and murine prothymosin alpha in vivo.人体内和小鼠体内原胸腺素α酰基磷酸酯的周转率。
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Prothymosin alpha in vivo contains phosphorylated glutamic acid residues.体内的前胸腺素α含有磷酸化的谷氨酸残基。
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Crystal structure of the nucleosome core particle at 2.8 A resolution.核小体核心颗粒的晶体结构，分辨率为2.8埃。
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Prothymosin alpha binds histones in vitro and shows activity in nucleosome assembly assay.前胸腺素α在体外与组蛋白结合，并在核小体组装试验中显示出活性。
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