Tabbarah Z A, Kohler R B, Wheat L J, Griep J A, White A
J Infect Dis. 1979 Nov;140(5):822-5. doi: 10.1093/infdis/140.5.822.
Specific antibodies have previously been found to impair detection of staphylococcal antigen by solid-phase radioimmunoassay. The inhibitory effect of heat-labile serum factors on the detection of antigen is now described. Heating of serum at 56 C for 30 min prior to addition of antigens of Pseudomonas aeruginosa or Staphylococcus aureus improved sensitivity by eight- to 32-fold. Heating was less effective when performed after addition of antigen. Treatment with zymosan reduced the inhibitory effect of serum, although less effectively than did preheating. Antigen in buffer was detected with 16 times less sensitivity in antibody-coated tubes exposed to fresh serum than in tubes exposed to heated serum. These findings suggest that complement factors can interact with antibody in coated tubes and at least some antigens and thereby inhibit detection of antigen. The finding that heat treatment improved the sensitivity of Ausria II -125 (Abbott Laboratories, North Chicago, Illinois) for hepatitis B surface antigen is of potential immediate clinical applicability.
此前已发现特异性抗体可通过固相放射免疫测定法损害葡萄球菌抗原的检测。现将描述不耐热血清因子对抗原检测的抑制作用。在加入铜绿假单胞菌或金黄色葡萄球菌抗原之前，将血清在56℃加热30分钟可使灵敏度提高8至32倍。在加入抗原后进行加热效果较差。用酵母聚糖处理可降低血清的抑制作用，但其效果不如预热。与暴露于加热血清的试管相比，暴露于新鲜血清的抗体包被试管中缓冲液中的抗原检测灵敏度降低了16倍。这些发现表明补体因子可与包被试管中的抗体以及至少某些抗原相互作用，从而抑制抗原检测。热处理提高了Ausria II -125（雅培实验室，伊利诺伊州北芝加哥）对乙型肝炎表面抗原的检测灵敏度，这一发现具有直接的潜在临床应用价值。
