人类和患有金黄色葡萄球菌性心内膜炎的免疫兔体内循环的葡萄球菌抗原：预先存在的抗体对检测的抑制作用。
Circulating staphylococcal antigen in humans and immune rabbits with endocarditis due to Staphylococcus aureus: inhibition of detection by preexisting antibodies.
作者信息
Wheat L J, Kohler R B, Tabbarah Z, White A
出版信息
J Infect Dis. 1979 Jul;140(1):54-61. doi: 10.1093/infdis/140.1.54.
A radioimmunoassay for staphylococcal antigen that had detected antigenemia in each of 12 nonimmune rabbits with staphylococcal endocarditis detected antigen in sera from one of nine humans and two of eight immune rabbits with Staphylococcus aureus endocarditis. Staphylococcal antigens could be detected at concentrations as low as 0.78 microgram/ml when diluted in normal rabbit serum, compared with 6.25 microgram/ml when diluted in normal human serum and greater than 25 microgram/ml when diluted in immune rabbit or human serum. Low titers of staphylococcal antibody were found in normal rabbit serum compared with immune rabbit and normal or immune human sera. Detection of staphylococcal antigen was inhibited when the antigen was diluted in the IgG and IgM fractions, but not in the albumin fraction, of normal human serum. This study demonstrated that antigenemia can be detected infrequently in patients and immune rabbits with staphylococcal endocarditis; staphylococcal antibodies inhibit detection of antigen, presumably through formation of immune complexes.
一种用于检测葡萄球菌抗原的放射免疫测定法，在12只患有葡萄球菌性心内膜炎的非免疫兔中均检测到了抗原血症，但在9名人类患者中的1人以及8只患有金黄色葡萄球菌性心内膜炎的免疫兔中的2只的血清中检测到了抗原。当在正常兔血清中稀释时，葡萄球菌抗原的检测浓度低至0.78微克/毫升，而在正常人类血清中稀释时为6.25微克/毫升，在免疫兔或人类血清中稀释时则大于25微克/毫升。与免疫兔血清以及正常或免疫人类血清相比，正常兔血清中发现的葡萄球菌抗体滴度较低。当抗原在正常人血清的IgG和IgM组分中稀释时，葡萄球菌抗原的检测受到抑制，但在白蛋白组分中则不受抑制。这项研究表明，在患有葡萄球菌性心内膜炎的患者和免疫兔中，抗原血症很少能被检测到；葡萄球菌抗体抑制抗原的检测，推测是通过形成免疫复合物实现的。
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