Wheat L J, Kohler R B, White A
J Infect Dis. 1978 Aug;138(2):174-80. doi: 10.1093/infdis/138.2.174.
To aid in the rapid diagnosis of staphylococcal infection, a solid-phase radioimmunoassay for Staphylococcus aureus antigen was developed and evaluated in rabbits with staphylococcal endocarditis. Test specimens containing antigen were added to polystyrene tubes coated with antibody to S. aureus. Antigens immobilized on the tube were detected by adding radiolabeled antibody to S. aureus. Sensitivity for antigen was 0.312 microgram/ml in buffer and 1.25 microgram/ml in 50% rabbit serum. Cross-reactions were not observed with antigens extracted from streptococci; however, antigen extracted from Staphylococcus epidermidis (which contained ribitol-teichoic acid) could also be detected at low concentrations. Antigen was detected in each of 12 rabbits with S. aureus endocarditis but not in control rabbits. This assay is sensitive, specific, reproducible, and capable of detecting antigens in the serum of rabbits with endocarditis.
为了有助于葡萄球菌感染的快速诊断，研发了一种用于检测金黄色葡萄球菌抗原的固相放射免疫测定法，并在患有葡萄球菌性心内膜炎的兔子身上进行了评估。将含有抗原的测试标本添加到涂有抗金黄色葡萄球菌抗体的聚苯乙烯管中。通过添加放射性标记的抗金黄色葡萄球菌抗体来检测固定在管上的抗原。在缓冲液中抗原的灵敏度为0.312微克/毫升，在50%兔血清中为1.25微克/毫升。未观察到与从链球菌中提取的抗原发生交叉反应；然而，从表皮葡萄球菌（含有核糖醇磷壁酸）中提取的抗原在低浓度下也能被检测到。在12只患有金黄色葡萄球菌性心内膜炎的兔子中均检测到了抗原，但在对照兔子中未检测到。该测定法灵敏、特异、可重复，并且能够检测心内膜炎兔子血清中的抗原。
