Sun Xue-Zhi, Takahashi Sentaro, Cui Chun, Inoue Minoru, Fukui Yoshihiro
Environmental and Toxicological Sciences Research Group, National Institute of Radiological Sciences, Anagawa, Inage-ku, Chiba 263-8555, Japan.
J Med Invest. 2002 Feb;49(1-2):35-9.
Distribution of calbindin-D28K immunoreactive cells in the primary motor area of the adult rat neocortex was studied in the present experiment. In the primary motor cortex, calbindin-D28K immunoreactivity was found in two populations of cortical neurons. One was composed of neurons heavily labeled with anti-calbindin antibody, which were present in two bands corresponding to cortical layers II-III, and V. The morphological types of these cells were varied; they had oval, fusiform or mutiangular somata. The proximal dendrites of the heavily stained cells showed that these cells were non-pyramidal neurons, and they were either bitufted or multipolar cells. The other was a weakly stained population, mainly concentrated in layers II and III, that also contained pyramidal neurons. In addition, one outstanding feature of the neuropil staining deep to layer II was the labeling of the long, vertically oriented bundles of immunoreactive processes. Such a distinct pattern of calbindin-D28K immunoreactive neurons in the primary motor cortex suggests a relatively high density of calcium channels exists in the superficial layers of the rat primary motor cortex.
在本实验中，研究了成年大鼠新皮质初级运动区中钙结合蛋白-D28K免疫反应性细胞的分布。在初级运动皮层中，钙结合蛋白-D28K免疫反应性存在于两类皮质神经元中。一类由用抗钙结合蛋白抗体重度标记的神经元组成，它们出现在与皮质层II-III和V相对应的两条带中。这些细胞的形态类型多样；它们具有椭圆形、梭形或多角形的胞体。重度染色细胞的近端树突表明这些细胞是非锥体神经元，它们要么是双簇状要么是多极细胞。另一类是染色较弱的群体，主要集中在层II和III，其中也包含锥体神经元。此外，层II深处神经毡染色的一个突出特征是长的、垂直定向的免疫反应性突起束的标记。初级运动皮层中钙结合蛋白-D28K免疫反应性神经元的这种独特模式表明大鼠初级运动皮层表层中存在相对高密度的钙通道。
