Anthony R M, Brown T J, French G L
NH Swellengrebel Laboratorium, voor Tropical Hygiene, Mibergdreef 39, 1105 AZ, Amsterdam, The Netherlands.
Expert Rev Mol Diagn. 2001 May;1(1):30-8. doi: 10.1586/14737159.1.1.30.
Near instantaneous detection of pathogens from clinical material, combined with simultaneous prediction of their antimicrobial resistance profiles, would revolutionize the impact of microbiology on the management of infection. Array-based assays allow a range of characteristics to be rapidly and simultaneously determined. At present these systems have found their primary role as research tools for the monitoring of mRNA expression in the form of DNA microarrays or 'chips'. As fabrication costs reduce and validated targeted arrays are developed, it is inevitable they will be used for more routine applications. Microfluidics offers the exciting possibility of combining purification, amplification and detection in a single disposable device; microarrays are particularly suitable for use within these systems. Arrays will become an important tool for clinical diagnostics.
从临床样本中近乎即时地检测病原体，并同时预测其抗菌药物耐药谱，将彻底改变微生物学对感染管理的影响。基于阵列的检测方法能够快速且同时测定一系列特征。目前，这些系统主要作为以DNA微阵列或“芯片”形式监测mRNA表达的研究工具。随着制造成本降低以及经过验证的靶向阵列的开发，它们不可避免地将用于更多常规应用。微流控技术提供了在单个一次性设备中结合纯化、扩增和检测的令人兴奋的可能性；微阵列特别适合在这些系统中使用。阵列将成为临床诊断的重要工具。
