用于分子生物学和诊断的新型芯片。

New chips for molecular biology and diagnostics.

作者信息

Zammatteo Nathalie, Hamels Sandrine, De Longueville Françoise, Alexandre Isabelle, Gala Jean-luc, Brasseur Francis, Remacle José

机构信息

Laboratory of Cellular Biochemistry, Facultés Universitaires Notre-Dame de la Paix, 61 Rue de Bruxelles, B-5000 Namur, Belgium.

出版信息

Biotechnol Annu Rev. 2002;8:85-101. doi: 10.1016/s1387-2656(02)08005-5.

DOI:10.1016/s1387-2656(02)08005-5

PMID:12436916

Abstract

The DNA chips are arrays of DNA probes immobilized on solid support for simultaneous identification of many target DNA sequences. DNA chips applied to diagnosis aims to detect genomic DNA or RNA after PCR amplification. This review provides an overview of DNA chip technology, focusing on diagnostic applications. A comparison between high density and low density microarrays is given showing that low density chips are more suitable for routine applications due to their simplicity, good reproducibility, easy data management and low cost.

摘要

DNA芯片是固定在固体支持物上的DNA探针阵列，用于同时鉴定多个靶DNA序列。应用于诊断的DNA芯片旨在通过聚合酶链反应（PCR）扩增后检测基因组DNA或RNA。本文综述了DNA芯片技术，重点介绍其在诊断方面的应用。文中对高密度和低密度微阵列进行了比较，结果表明，低密度芯片因其操作简单、重复性好、数据管理方便且成本低，更适合常规应用。