Paratore Christian, Hagedorn Lilian, Floris Julien, Hari Lisette, Kléber Maurice, Suter Ueli, Sommer Lukas
Institute of Cell Biology, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich.
Int J Dev Biol. 2002 Jan;46(1):193-200.
Multipotent stem cells must generate various differentiated cell types in correct number and sequence during neural development. In the peripheral nervous system (PNS), this involves the formation of postmigratory progenitor cell types which maintain multipotency and are able to give rise to neural and non-neural cells in response to instructive growth factors. We propose that fate restrictions in such progenitor cells are controlled by the combinatorial interaction of different extracellular signals, including community effects in response to both neurogenic and gliogenic factors. In addition, distinct progenitor cell types display intrinsic differences which modulate their response to the extracellular environment. Thus, a progenitor cell is apparently able to integrate multiple intrinsic and extrinsic cues and thereby to choose fates appropriate for its location. Fate analysis of genetically modified progenitor cells will help to identify the molecules involved. This approach appears promising given the identification of multipotent progenitor cells from the mouse PNS and the availability of genetics in the mouse system.
多能干细胞在神经发育过程中必须以正确的数量和顺序产生各种分化的细胞类型。在周围神经系统（PNS）中，这涉及到迁移后祖细胞类型的形成，这些祖细胞保持多能性，并能够在有指导性的生长因子作用下产生神经细胞和非神经细胞。我们提出，此类祖细胞中的命运限制是由不同细胞外信号的组合相互作用控制的，包括对神经源性和胶质源性因子反应中的群体效应。此外，不同的祖细胞类型表现出内在差异，这些差异调节它们对细胞外环境的反应。因此，祖细胞显然能够整合多种内在和外在线索，从而选择适合其位置的命运。对基因改造的祖细胞进行命运分析将有助于识别其中涉及的分子。鉴于从小鼠PNS中鉴定出多能祖细胞以及小鼠系统中遗传学的可用性，这种方法似乎很有前景。
