Mujtaba T, Mayer-Proschel M, Rao M S
Department of Neurobiology and Anatomy, Department of Oncological Sciences, University of Utah Medical School, 50 North Medical Drive, Salt Lake City, Utah, 84132, USA.
Dev Biol. 1998 Aug 1;200(1):1-15. doi: 10.1006/dbio.1998.8913.
Cultured spinal cord neuroepithelial (NEP) cells can differentiate into neurons, oligodendrocytes and astrocytes and are morphologically and antigenically distinct from neural crest stem cells (NCSCs) that generate the PNS. NEP cells, however, can generate p75/nestin-immunoreactive cells that are morphologically and antigenically similar to previously characterized NCSCs. NEP-derived p75-immunoreactive cells differentiate into peripheral neurons, smooth muscle, and Schwann cells in mass and clonal culture. Clonal analysis of NEP cells demonstrates that a common NEP progenitor cell generated both CNS and PNS phenotypes. Differentiation into NCSCs was promoted by BMP-2/4 and differentiation did not require cells to divide, indicating that BMP played an instructive role in the differentiation process. Thus, individual NEP cells are multipotent and can differentiate into most major types of cell in the CNS and PNS and that PNS differentiation involves a transition from a NEP stem to another more limited, p75-immunoreactive, neural crest stem cell.
培养的脊髓神经上皮（NEP）细胞可分化为神经元、少突胶质细胞和星形胶质细胞，在形态和抗原性上与产生外周神经系统（PNS）的神经嵴干细胞（NCSCs）不同。然而，NEP细胞可产生p75/巢蛋白免疫反应性细胞，这些细胞在形态和抗原性上与先前鉴定的NCSCs相似。在大规模和克隆培养中，源自NEP的p75免疫反应性细胞可分化为外周神经元、平滑肌和雪旺细胞。对NEP细胞的克隆分析表明，一个共同的NEP祖细胞可产生中枢神经系统（CNS）和PNS表型。BMP-2/4促进向NCSCs的分化，且分化不需要细胞分裂，这表明BMP在分化过程中起指导作用。因此，单个NEP细胞具有多能性，可分化为CNS和PNS中的大多数主要细胞类型，并且PNS分化涉及从NEP干细胞向另一种更有限的、p75免疫反应性的神经嵴干细胞的转变。
