Matsumoto M, Kotani M, Fujita A, Higa S, Kishimoto T, Suemura M, Tanaka T
Research and Development Center, Sunstar Incorporation, Osaka, Japan.
Br J Dermatol. 2002 Feb;146(2):221-7. doi: 10.1046/j.1365-2133.2002.04557.x.
We have previously shown that persimmon leaf extract and its major flavonoid constituent, astragalin, inhibited histamine release by basophils and that oral administration of these substances prior to the onset into an atopic dermatitis (AD) model mouse, NC/Nga, prevented development of dermatitis.
This study was designed to assess the clinical therapeutic effect of persimmon leaf extract and astragalin in NC/Nga mice suffering from dermatitis and the dose-response preventive effects of persimmon leaf extract on dermatitis and transepidermal water loss (TEWL).
The efficacy of persimmon leaf extract or astragalin in NC/Nga mice was judged by measurement of skin severity, scratching behaviour, serum IgE levels or TEWL.
Oral administration of persimmon leaf extract (250 mg kg(-1)) or astragalin (1.5 mg kg-1) for 4 weeks into NC/Nga mice with overt dermatitis resulted in a decrease in the severity of the condition. The preventive effect of persimmon leaf extract on the dermatitis was dose-dependent and continuous intake of persimmon leaf extract significantly decreased its onset and development. In addition, TEWL was also suppressed at a persimmon leaf extract dose of 250 mg kg(-1). No significant adverse reaction by these substances could be observed.
These observations suggest that persimmon leaf extract or the flavonoid astragalin may be alternative substances for the management of AD.
我们之前已经表明，柿叶提取物及其主要黄酮成分紫云英苷可抑制嗜碱性粒细胞释放组胺，并且在特应性皮炎（AD）模型小鼠NC/Nga发病前口服这些物质可预防皮炎的发展。
本研究旨在评估柿叶提取物和紫云英苷对患有皮炎的NC/Nga小鼠的临床治疗效果，以及柿叶提取物对皮炎和经表皮水分流失（TEWL）的剂量反应预防作用。
通过测量皮肤严重程度、搔抓行为、血清IgE水平或TEWL来判断柿叶提取物或紫云英苷对NC/Nga小鼠的疗效。
对患有明显皮炎的NC/Nga小鼠口服柿叶提取物（250 mg·kg⁻¹）或紫云英苷（1.5 mg·kg⁻¹）4周后，病情严重程度降低。柿叶提取物对皮炎的预防作用呈剂量依赖性，持续摄入柿叶提取物可显著减少其发病和发展。此外，在柿叶提取物剂量为250 mg·kg⁻¹时，TEWL也受到抑制。未观察到这些物质有明显的不良反应。
这些观察结果表明，柿叶提取物或黄酮类紫云英苷可能是治疗AD的替代物质。
