Milanezi F, Pereira E M, Ferreira F V, Leitão D, Schmitt F C
Institute of Pathology and Molecular Immunology of Porto University (IPATIMUP), Rua Dr Roberto Frias, s/n 4200 Porto, Portugal.
Histopathology. 2001 Dec;39(6):578-83. doi: 10.1046/j.1365-2559.2001.01309.x.
To evaluate the expression of CD99/MIC-2 surface protein in invasive breast carcinomas and demonstrate whether or not there is a relationship with tumour phenotype.
Thirty-five invasive breast carcinomas, including five metaplastic carcinomas, were stained with CD99 primary antibodies using standard protocols based on streptavidin-biotin-peroxidase method. Four out of five metaplastic carcinomas expressed CD99/MIC-2 protein, three of them were matrix-producing carcinomas. From the other 30 cases, only an invasive apocrine carcinoma was positive. There was no statistical correlation between CD99 expression and the parameters analysed (histological typing and grading, proliferative index and nodal status).
CD99/MIC-2 is expressed in breast carcinomas, especially in the matrix-producing variant of metaplastic carcinomas, which impairs its use as a marker to differentiate metaplastic carcinomas from primary and metastatic sarcomas of the breast. It seems to have no prognostic implications. However, phenotype similarities with other chondromyxoid tumours that also express the protein, like mesenchymal chondrosarcomas, suggest a relationship between MIC-2 reactivity and morphological differentiation.
评估CD99/MIC-2表面蛋白在浸润性乳腺癌中的表达，并证明其与肿瘤表型是否存在关联。
采用基于链霉亲和素-生物素-过氧化物酶法的标准方案，用CD99一抗对35例浸润性乳腺癌（包括5例化生性癌）进行染色。5例化生性癌中有4例表达CD99/MIC-2蛋白，其中3例为产基质癌。在其他30例病例中，只有1例浸润性大汗腺癌呈阳性。CD99表达与所分析的参数（组织学类型和分级、增殖指数和淋巴结状态）之间无统计学相关性。
CD99/MIC-2在乳腺癌中表达，尤其是在化生性癌的产基质变体中，这削弱了其作为区分化生性癌与乳腺原发性和转移性肉瘤的标志物的用途。它似乎没有预后意义。然而，与其他也表达该蛋白的软骨黏液样肿瘤（如间叶性软骨肉瘤）的表型相似性表明，MIC-2反应性与形态学分化之间存在关联。
