Vranic Semir, Gurjeva Olga, Frkovic-Grazio Snjezana, Palazzo Juan, Tawfik Ossama, Gatalica Zoran
Department of Pathology, Clinical Center of the University of Sarajevo, Bosnia and Herzegovina.
Appl Immunohistochem Mol Morphol. 2011 Oct;19(5):413-6. doi: 10.1097/PAI.0b013e3182143399.
Insulin-like growth factor-II mRNA-binding protein 3 (IMP3) is a member of the insulin-like growth factor-II signaling pathway, and has recently been described as a biomarker of basal-like breast carcinomas. This study explored IMP3 expression in adenoid cystic carcinomas of the breast, a special type of basal-like, triple-negative (estrogen receptor/progesterone receptor/human epidermal growth factor receptor 2/neu protein negative) carcinoma and compared it with a group of apocrine carcinomas, which are an example of estrogen receptor/progesterone receptor negative, special type of breast carcinoma. Eighteen breast adenoid cystic carcinomas (16 primary and 2 corresponding metastases) and 18 apocrine carcinomas (16 invasive and 2 in situ) were evaluated for the expression of IMP3 protein using immunohistochemical method. A cut-off value for IMP3 positivity was set at 10%. Thirteen of 16 (81.3%) primary adenoid cystic carcinomas overexpressed IMP3 protein, predominantly in membranous distribution. The mean percentage of positive cells among primary adenoid cystic carcinomas was 50%. Both metastatic adenoid cystic carcinomas also strongly overexpressed IMP3 protein (70% and 80% of the tumor cells, respectively). In contrast, only 4 of 16 invasive apocrine carcinomas (25%) exhibited IMP3 positivity with significantly lower percentage of positive cells (27%, P<0.001). Two in-situ apocrine carcinomas were negative. Our results indicate that IMP3 may be an additional basal-type marker in breast carcinoma whose expression can be occasionally seen in other types of breast carcinomas such as apocrine type.
胰岛素样生长因子-II mRNA结合蛋白3（IMP3）是胰岛素样生长因子-II信号通路的成员，最近被描述为基底样乳腺癌的生物标志物。本研究探讨了IMP3在乳腺腺样囊性癌中的表达，乳腺腺样囊性癌是一种特殊类型的基底样三阴（雌激素受体/孕激素受体/人表皮生长因子受体2/neu蛋白阴性）癌，并将其与一组大汗腺癌进行比较，大汗腺癌是雌激素受体/孕激素受体阴性的特殊类型乳腺癌的一个例子。采用免疫组化方法评估了18例乳腺腺样囊性癌（16例原发性和2例相应转移灶）和18例大汗腺癌（16例浸润性和2例原位癌）中IMP3蛋白的表达。将IMP3阳性的临界值设定为10%。16例原发性腺样囊性癌中有13例（81.3%）IMP3蛋白过表达，主要呈膜性分布。原发性腺样囊性癌中阳性细胞的平均百分比为50%。两个转移性腺样囊性癌也强烈过表达IMP3蛋白（分别为70%和80%的肿瘤细胞）。相比之下，16例浸润性大汗腺癌中只有4例（25%）表现出IMP3阳性，阳性细胞百分比显著较低（27%，P<0.001）。2例原位大汗腺癌为阴性。我们的结果表明，IMP3可能是乳腺癌中另一种基底型标志物，其表达偶尔可见于其他类型的乳腺癌，如大汗腺型。
