Bayer Ilana M, Caniggia Isabella, Adamson S Lee, Langille B Lowell
Toronto General Research Institute, University Health Network, Toronto, ON, Canada.
Cell Tissue Res. 2002 Mar;307(3):303-13. doi: 10.1007/s00441-002-0512-4. Epub 2002 Feb 19.
Vasa vasorum are important sources of oxygen and nutrients to vascular tissues and their proliferation influences the pathogenesis of arterial disease; however, the regulation of their growth is poorly understood partly because of a lack of appropriate experimental models. We cuffed common carotid arteries of rabbits with segments of the contralateral carotid artery, a procedure that resulted in rapid and extensive elaboration of adventitial vasa vasorum and connective tissue. Endothelium-lined microvessels were observed at 1 week but vessels as large as 300 microm with an organizing media were common by 3 weeks. These vasa vasorum arose primarily from the vascular supply to contiguous tissues, but also from the carotid artery. This angiogenesis was accompanied by increased expression of the angiogenic factor, vascular endothelial growth factor (VEGF), in the invading connective tissue cells and increased expression of the transcriptional regulator of VEGF, hypoxia-inducible factor-1alpha (HIF-1alpha), in these connective tissues and in the cuffing artery. These findings are consistent with the hypothesis that upregulation of HIF-1alpha and VEGF expression drives angiogenesis of vasa vasorum in this model. This simple model may be amenable to the study of the development and elaboration of vasa vasorum, especially in the context of vascular pathologies.
血管滋养血管是血管组织氧气和营养物质的重要来源，其增殖影响动脉疾病的发病机制；然而，由于缺乏合适的实验模型，对其生长的调控了解甚少。我们用对侧颈动脉段对家兔的颈总动脉进行套扎，该操作导致外膜血管滋养血管和结缔组织迅速大量增生。1周时观察到有内皮衬里的微血管，但到3周时，直径达300微米且有组织化中膜的血管很常见。这些血管滋养血管主要起源于相邻组织的血管供应，但也来自颈动脉。这种血管生成伴随着血管生成因子血管内皮生长因子（VEGF）在侵入的结缔组织细胞中的表达增加，以及VEGF转录调节因子缺氧诱导因子-1α（HIF-1α）在这些结缔组织和套扎动脉中的表达增加。这些发现与以下假设一致，即在该模型中HIF-1α和VEGF表达的上调驱动了血管滋养血管的血管生成。这个简单的模型可能适用于研究血管滋养血管的发育和增生，特别是在血管病理情况下。
