Caballero T, Alonso A, De Miguel S, Martín-Esteban M, Varga B, Pascual C Y, López-Serrano M C
Allergy Service, University Hospital La Paz, Madrid, Spain.
Allergy. 2002 Mar;57(3):254-7. doi: 10.1034/j.1398-9995.2002.03322.x.
Thiomucase is a mucopolysaccharidase obtained from ovine tissues mainly used to facilitate the diffusion of local anaesthetics and in the treatment of cellulitis. A patient with an anaphylaxis in relation to the intramuscular administration of Thiomucase is reported.
To investigate Thiomucase allergens and their possible relationship with dander allergens and animal albumins.
Skin prick tests (SPT) and serum-specific IgE were performed with Thiomucase and danders. Thiomucase SDS-PAGE immunoblotting was performed in order to study allergens. RAST/CAP inhibition and SDS-PAGE immunoblotting inhibition were carried out to study the cross-reactivity.
Skin prick tests (SPT) were positive to Thiomucase, animal dander (cat, dog, sheep, other), bovine serum albumin (BSA), and echinococcus. Specific IgE was also positive to Thiomucase, animal dander (cat, dog, sheep, other), BSA and echinococcus. In the RAST-CAP inhibition assays BSA was nearly completely inhibited by Thiomucase, Thiomucase was partially inhibited by BSA and cat and Echinococcus granulosus was partially inhibited by sheep and Thiomucase. In the Thiomucase SDS-PAGE immunoblotting several proteins fixed IgE, ranging from 20 kDa to > 94 kDa, the strongest with 43 kDa. The IgE fixation to BSA, cat and sheep in the SDS-PAGE immunoblotting was completely inhibited by the preincubation of the serum with Thiomucase.
An IgE-mediated anaphylaxis to Thiomucase is documented. Multiple allergens are recognized in Thiomucase by the patient serum, the main with 43 kDa. Partial cross-reactivity with BSA, cat dander and sheep dander is documented.
硫黏液酶是一种从绵羊组织中提取的黏多糖酶，主要用于促进局部麻醉剂的扩散以及治疗蜂窝织炎。本文报道了1例因肌内注射硫黏液酶而发生过敏反应的患者。
研究硫黏液酶过敏原及其与皮屑过敏原和动物白蛋白之间的可能关系。
用硫黏液酶和皮屑进行皮肤点刺试验（SPT）和血清特异性IgE检测。进行硫黏液酶SDS-PAGE免疫印迹以研究过敏原。开展RAST/CAP抑制试验和SDS-PAGE免疫印迹抑制试验以研究交叉反应性。
皮肤点刺试验对硫黏液酶、动物皮屑（猫、狗、绵羊、其他）、牛血清白蛋白（BSA）和棘球绦虫呈阳性。特异性IgE对硫黏液酶、动物皮屑（猫、狗、绵羊、其他）、BSA和棘球绦虫也呈阳性。在RAST-CAP抑制试验中，硫黏液酶几乎完全抑制了BSA，BSA部分抑制了硫黏液酶，猫和细粒棘球绦虫被绵羊和硫黏液酶部分抑制。在硫黏液酶SDS-PAGE免疫印迹中，几种蛋白质固定了IgE，分子量范围从20 kDa至>94 kDa，最强的为43 kDa。在SDS-PAGE免疫印迹中，血清与硫黏液酶预孵育后，对BSA、猫和绵羊的IgE固定被完全抑制。
记录了1例IgE介导的对硫黏液酶的过敏反应。患者血清识别出硫黏液酶中的多种过敏原，主要为43 kDa的过敏原。记录了与BSA、猫皮屑和绵羊皮屑的部分交叉反应性。
