Cabañas R, López-Serrano M C, Carreira J, Ventas P, Polo F, Caballero M T, Contreras J, Barranco P, Moreno-Ancillo A
Allergy Service, Hospital La Paz, Madrid, Spain.
J Investig Allergol Clin Immunol. 2000 Mar-Apr;10(2):71-7.
Different allergenic proteins have been involved in cross-reactivity among animals. Albumins seem to be cross-sensitizing allergenic components. The aim of this study was to assess the importance of albumin as a cross-reactive allergen in patients sensitized to cat, dog and horse. One hundred and seventeen patients sensitized to cat were tested for IgE reactivity using skin prick tests and RAST assays with cat, dog and horse hair/dander extracts and their purified albumin extracts. RAST-inhibition studies were carried out to assess cross-reactivity among cat, dog and horse and among their purified albumins. It was found that 22% of patients exhibited specific IgE to cat albumin; 41% of patients sensitized to cat were also sensitized to dog and horse. Out of these patients, 21% had IgE to three albumins and 17% to two. Reciprocal inhibitions were observed among cat, dog and horse albumins and also among cat, dog and horse hair/dander extracts, using in the latter experiment sera from patients not sensitized to albumins. IgE binding to horse extract was inhibited 30% by its homologous albumin and IgE binding to cat and dog extracts in almost 15% by their respective albumins. It was concluded that albumins from these three animals share some epitopes that account for the cross-reactivity observed in around one-third of patients sensitized to cat, dog and horse. Nevertheless, more than 50% of specific IgE that cross-reacts among these three animals is directed to allergens other than albumin.
不同的变应原性蛋白质参与了动物之间的交叉反应。白蛋白似乎是交叉致敏的变应原成分。本研究的目的是评估白蛋白作为猫、狗和马致敏患者交叉反应性变应原的重要性。对117名猫致敏患者进行了皮肤点刺试验和RAST检测，使用猫、狗和马的毛发/皮屑提取物及其纯化的白蛋白提取物检测IgE反应性。进行RAST抑制研究以评估猫、狗和马及其纯化白蛋白之间的交叉反应性。结果发现，22%的患者对猫白蛋白表现出特异性IgE；41%的猫致敏患者也对狗和马致敏。在这些患者中，21%对三种白蛋白有IgE，17%对两种白蛋白有IgE。在猫、狗和马的白蛋白之间以及猫、狗和马的毛发/皮屑提取物之间观察到相互抑制，在后者的实验中使用未对白蛋白致敏患者的血清。马提取物与IgE的结合被其同源白蛋白抑制30%，猫和狗提取物与IgE的结合被其各自的白蛋白抑制近15%。得出的结论是，这三种动物的白蛋白共享一些表位，这解释了在大约三分之一的猫、狗和马致敏患者中观察到的交叉反应性。然而，在这三种动物之间交叉反应的特异性IgE中，超过50%是针对白蛋白以外的变应原。
