Araujo F G, Slifer T L, Remington J S
Research Institute, Palo Alto Medical Foundation, Palo Alto, CA 94301, USA.
Clin Microbiol Infect. 2002 Jan;8(1):26-30. doi: 10.1046/j.1469-0691.2002.00374.x.
To determine the effect of moxifloxacin on secretion of cytokines by human monocytes stimulated with lipopolysaccharide (LPS) or Pansorbin.
Monocytes obtained from 10 healthy volunteer donors were stimulated with LPS or Pansorbin and exposed or not to different concentrations of the fluoroquinolone antibiotic moxifloxacin. At 3, 6 and 24 h, the amounts of interleukin-1alpha (IL-1alpha), IL-1beta, IL-6, IL-10, IL-12 (p70) and tumour necrosis factor-alpha (TNF-alpha) were measured in the supernatants of the monocyte cultures using enzyme-linked immunosorbent assay.
Stimulation of human monocytes with either LPS or Pansorbin resulted in a significant increase in secretion of each of the cytokines examined. Treatment of LPS-stimulated monocytes with moxifloxacin significantly inhibited (P < 0.01) secretion of IL-1alpha by monocytes of each of 10 human donors; the secretion of TNF-alpha was significantly inhibited (P < 0.01) in monocytes from six of 10 donors. In general there was a trend towards inhibition of secretion of IL-6, IL-10 and IL-12 (p70), but the inhibitory effect was not statistically significant. Secretion of cytokines by Pansorbin-stimulated monocytes was not significantly inhibited by moxifloxacin.
Moxifloxacin has immunomodulatory activity through its capacity to alter the secretion of IL-1alpha and TNF-alpha by human monocytes.
确定莫西沙星对脂多糖（LPS）或泛福舒刺激的人单核细胞分泌细胞因子的影响。
从10名健康志愿者供体获取的单核细胞用LPS或泛福舒刺激，并暴露于不同浓度的氟喹诺酮类抗生素莫西沙星或不暴露。在3、6和24小时时，使用酶联免疫吸附测定法测量单核细胞培养上清液中白细胞介素-1α（IL-1α）、IL-1β、IL-6、IL-10、IL-12（p70）和肿瘤坏死因子-α（TNF-α）的量。
用LPS或泛福舒刺激人单核细胞导致所检测的每种细胞因子的分泌显著增加。用莫西沙星处理LPS刺激的单核细胞显著抑制（P<0.01）10名人类供体中每名供体的单核细胞分泌IL-1α；10名供体中有6名供体的单核细胞中TNF-α的分泌被显著抑制（P<0.01）。总体而言，存在抑制IL-6、IL-10和IL-12（p70）分泌的趋势，但抑制作用无统计学意义。莫西沙星未显著抑制泛福舒刺激的单核细胞分泌细胞因子。
莫西沙星具有免疫调节活性，通过其改变人单核细胞分泌IL-1α和TNF-α的能力来实现。
