Reveneau Nathalie, Geoffroy Marie Claude, Locht Camille, Chagnaud Patrice, Mercenier Annick
Laboratoire de Bactériologie des Ecosystèmes, INSERM IFR17, Institut Pasteur de Lille, 1 rue du Professeur Calmette, F-59019 Lille Cedex, France.
Vaccine. 2002 Mar 15;20(13-14):1769-77. doi: 10.1016/s0264-410x(02)00027-0.
Lactobacillus plantarum NCIMB8826 was selected as a bacterial carrier for the development of live mucosal vaccines. This strain was reported to display interesting pharmaco-kinetic properties when fed to human volunteers and is also able to persist in the mouse intestine. The non-toxic C fragment of tetanus toxin (TTFC) was used as a model antigen. Recombinant strains producing TTFC in three cellular locations, intracellular, secreted or cell-surface exposed were compared to each other by immunizing mice through the subcutaneous, intranasal and intragastric routes. The three types of constructs were able to induce strong specific immune responses against TTFC by all routes tested. While cell-surface presentation required lower antigen doses to be immunogenic, the highest IgG serum antibody titers were obtained with the strain producing large amounts of TTFC in the cytoplasm.
植物乳杆菌NCIMB8826被选为开发活黏膜疫苗的细菌载体。据报道，该菌株在喂食给人类志愿者时表现出有趣的药代动力学特性，并且还能够在小鼠肠道中持续存在。破伤风毒素的无毒C片段（TTFC）被用作模型抗原。通过皮下、鼻内和胃内途径免疫小鼠，比较了在细胞内、分泌型或细胞表面表达三种细胞定位产生TTFC的重组菌株。所有测试途径中，这三种构建体均能够诱导针对TTFC的强烈特异性免疫反应。虽然细胞表面呈递所需的免疫原性抗原剂量较低，但在细胞质中产生大量TTFC的菌株获得了最高的IgG血清抗体滴度。
