Reinberg A, Lagoguey M, Chauffournier M, Cesselin F
Ann Endocrinol (Paris). 1975 Jan-Feb;36(1):44-5.
Every other month, during the course of 14 months, the circadian rhythm in plasma testosterone (among others variables) of 5 healthy males (26 to 32 years) was documented. Subjects' synchronization : light-on at 07 +/- 1 hr; light-off at 23hr +/- 1.5 hr all the year long. Blood sampling 4-hourly, at fixed clock hours during 28 hrs, for radioimmunologic testosterone determinations. The cosinor method was used for statistical analyses. An annual rhythm in the testosterone 24-hr mean is detected (p less than 0.025) with a peak in October (from 16 July to 26 December, with 95% confidence limits). Annual mean (+/- 1 SE) - 759 (+/- 26)ng/100 ml. Peak-through difference = 107 ng/100 ml (from 106 to 290 with 95% confidence limits). The timing of the testosterone circadian peak, in the 24 hr-scale varies also in the course of the year. The peak is found around 08(00) in May and around 14(00) in November, this phase-shift being statistically significant (p less than 0.05). The sexual activity (daily self-recording) also has an annual rhythm with a peak in October.
在14个月的时间里，每隔一个月记录5名健康男性（26至32岁）血浆睾酮（以及其他变量）的昼夜节律。受试者的同步情况：全年早上7点±1小时开灯；晚上23点±1.5小时关灯。每4小时在固定的时钟时间进行采血，持续28小时，用于放射免疫法测定睾酮。采用余弦分析方法进行统计分析。检测到睾酮24小时均值存在年度节律（p<0.025），10月份出现峰值（7月16日至12月26日，95%置信区间）。年度均值（±1标准误）-759（±26）ng/100ml。峰值与谷值之差=107ng/100ml（95%置信区间为106至290）。睾酮昼夜峰值在24小时尺度上的时间在一年中也有所变化。5月份峰值出现在08(00)左右，11月份出现在14(00)左右，这种相位变化具有统计学意义（p<0.05）。性活动（每日自我记录）也有年度节律，10月份出现峰值。
