Reinberg A, Dupont W, Touitou Y, Lagoguey M, Bourgeois P, Touitou C, Muriaux G, Przyrowsky D, Guillemant S, Guillemant J, Brière L, Zeau B
Chronobiologia. 1981 Jan-Mar;8(1):11-31.
The aim of the investigation was to study the effects of ACTH 1-17 on plasma testosterone, plasma aldosterone as well as on both plasma and urinary electrolytes (K, Na, Mg and Ca) in healthy young adult males with regard to the time (clock hours) at which this polypeptide was injected. Eight healthy adults (males from 28 to 30 years) volunteered for the study. The were synchronized with a diurnal activity from 0700 to midnight and a nocturnal rest. Each week, during 6 consecutive weeks (January 19 to February 25, 1980) a 3-day test was performed on Saturday, Sunday and Monday. On Sundays 3 control-tests and the 3 ACTH-tests were programmed during which either saline or 100 microgram ACTH 1-17 were injected i.m. at respectively 0700, 1400 and 2100. During each 3 day-test period (72 h) the urinary excretion of K, Na, Mg and Ca was determined every 4 h at fixed clock hours. In addition, on Sundays, venous blood was sampled prior to control or ACTH injections at respectively 0700, 1400 and 2100 and 20, 40, 60, 90, 120, 150 and 180 min thereafter. Plasma testosterone, aldosterone (radioimmunoassays) K, Na (flame photometry), Mg and Ca (photocolorimetric methods) were determined in the collected samples. Both conventional and cosinor methods were used for statistical analyses. The injection of ACTH at 0700 was followed by a clear and statistically significant rise of plasma testosterone. No change with regard to control occurred when ACTH was injected at either 1400 or at 2100. A statistically significant rise of plasma aldosterone was observed after each of the ACTH injections. However, the highest plasma aldosterone level was reached when ACTH was administered at 1400 and the lowest level at 2100. ACTH-induced changes in plasma electrolytes were either nil (for Na and Ca) or small (for K and Mg). A more or less important increase of urinary K occurred after the ACTH injection at each of the 3 considered times. The highest values of excreted K occurred after the injection of ACTH at 0700, without shift of the acrophase. In contrast, injections of ACTH at 1400 and 2100 induced a dramatic alteration of the K rhythms. ACTH induced an important fall in the Na urinary excretion. This fall was the greatest when ACTH was injected at 1400. Na rhythm alterations also occurred, particularly after ACTH injections at 2100. However, this effect was less pronounced after ACTH injection at 0700 than at other considered time points. The urinary amount of excreted Ca did not seem to be affected by ACTH. Rhythm alterations occurred after ACTH injections at 1400 and 2100. Peaks of plasma testosterone, plasma aldosterone as well as plasma cortisol (reported in a previous paper) resulting from ACTH stimulation coincided in time with the acrophase of the physiological circadian rhythm in plasma levels of these hormones...
本研究旨在探讨在健康年轻成年男性中，注射促肾上腺皮质激素1-17（ACTH 1-17）的时间（时钟小时数）对血浆睾酮、血浆醛固酮以及血浆和尿液电解质（钾、钠、镁和钙）的影响。八名健康成年人（年龄在28至30岁之间的男性）自愿参与该研究。他们的日常活动与从07:00至午夜的日间活动以及夜间休息同步。在连续6周（1980年1月19日至2月25日）的每周中，在周六、周日和周一进行为期3天的测试。在周日安排了3次对照测试和3次ACTH测试，期间分别在07:00、14:00和21:00肌肉注射生理盐水或100微克ACTH 1-17。在每个为期3天的测试期间（72小时），每隔4小时在固定的时钟时间测定钾、钠、镁和钙的尿排泄量。此外，在周日，分别在07:00、14:00和21:00以及之后的20、40、60、90、120、150和180分钟，在对照或ACTH注射前采集静脉血样。测定所采集样本中的血浆睾酮、醛固酮（放射免疫分析法）、钾、钠（火焰光度法）、镁和钙（比色法）。采用传统方法和余弦分析法进行统计分析。07:00注射ACTH后，血浆睾酮出现明显且具有统计学意义的升高。14:00或21:00注射ACTH时，与对照相比无变化。每次注射ACTH后，血浆醛固酮均出现具有统计学意义的升高。然而，14:00注射ACTH时血浆醛固酮水平最高，21:00时最低。ACTH诱导的血浆电解质变化要么为零（钠和钙），要么较小（钾和镁）。在三个考虑的时间点每次注射ACTH后，尿钾均出现或多或少的显著增加。07:00注射ACTH后排泄钾的最高值出现，且峰值相位未发生偏移。相比之下，14:00和21:00注射ACTH导致钾节律发生显著改变。ACTH导致尿钠排泄显著减少。14:00注射ACTH时这种减少最为明显。钠节律也发生改变，尤其是在21:00注射ACTH后。然而，07:00注射ACTH后的这种效应不如在其他考虑的时间点明显。尿钙排泄量似乎不受ACTH影响。14:
