Reinberg A, Lagoguey M, Chauffournier J M, Cesselin F
Acta Endocrinol (Copenh). 1975 Dec;80(4):732-4. doi: 10.1530/acta.0.0800732.
Every other month, during the course of 14 months, the circadian rhythm in plasma testosterone (among others variables) of 5 healthy males (26 to 32 years) was documented. Subjects' synchronization: light-on at 7 +/- 1 h; light-off at 23 +/- 1.5 h during the whole year. Blood sampling 4-hourly, at fixed times during 28 h, for radioimmunological testosterone determinations. The cosinor method was used for statistical analyses. An annual rhythm in the testosterone 24-h mean is detected (P less than 0.025) with a peak in October (from 16 July to 26 December, with 95% confidence limits). Annual mean (+/-1 se)= 759 (+/- 26) ng/100 ml. Peaktrough difference=197 ng/100 ml (from 106 to 290 with 95% confidence limits). The timing of the testosterone circadian peak, in the 24-h-scales also varies during the course of the year. The peak is found around 8 h in May and around 14 h in November, this phase-shift being statistically significant (P less than 0.05).
在14个月的时间里，每隔一个月对5名健康男性（26至32岁）的血浆睾酮（以及其他变量）的昼夜节律进行记录。受试者的同步情况：全年开灯时间为7±1小时；关灯时间为23±1.5小时。每4小时在固定时间进行一次采血，持续28小时，用于放射免疫法测定睾酮。采用余弦分析方法进行统计分析。检测到睾酮24小时均值存在年度节律（P<0.025），10月份出现峰值（7月16日至12月26日，95%置信区间）。年度均值（±1标准误）=759（±26）ng/100ml。峰谷差值=197ng/100ml（95%置信区间为106至290）。在一年中，睾酮昼夜节律峰值在24小时尺度上的时间也有所变化。5月份峰值出现在8时左右，11月份出现在14时左右，这种相位变化具有统计学意义（P<0.05）。
