Blanchard Emmanuelle, Hourioux Christophe, Brand Denys, Ait-Goughoulte Malika, Moreau Alain, Trassard Sylvie, Sizaret Pierre-Yves, Dubois Frederic, Roingeard Philippe
Laboratoire de Virologie, Faculté de Médecine et Centre Hospitalier Universitaire, 2 bis Boulevard Tonnellé, 37032 Tours, France.
J Virol. 2003 Sep;77(18):10131-8. doi: 10.1128/jvi.77.18.10131-10138.2003.
In the absence of a hepatitis C virus (HCV) culture system, the use of a Semliki Forest virus replicon expressing genes encoding HCV structural proteins that assemble into HCV-like particles provides an opportunity to study HCV morphogenesis. Using this system, we showed that the HCV core protein constitutes the budding apparatus of the virus and that its targeting to the endoplasmic reticulum by means of the signal sequence of E1 protein is essential for budding. In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis.
由于缺乏丙型肝炎病毒（HCV）培养系统，利用表达编码可组装成HCV样颗粒的HCV结构蛋白基因的辛德毕斯病毒复制子，为研究HCV形态发生提供了一个机会。利用该系统，我们发现HCV核心蛋白构成了病毒的出芽装置，并且通过E1蛋白的信号序列将其靶向内质网对于出芽至关重要。此外，发现HCV核心蛋白序列中第111位的天冬氨酸对于病毒组装至关重要，这证明了该系统在定位对HCV形态发生至关重要的氨基酸方面的有用性。
