Dhepakson Panadda, Mori Yasuko, Jiang Yun Bao, Huang Hong Lan, Akkapaiboon Pilailuk, Okuno Toshiomi, Yamanishi Koichi
Department of Microbiology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan1.
Department of Bacteriology, Hyogo College of Medicine, Hyogo, Japan2.
J Gen Virol. 2002 Apr;83(Pt 4):847-854. doi: 10.1099/0022-1317-83-4-847.
The characterization is reported of the human herpesvirus-6B (HHV-6B) rep/U94 gene, which is a homologue of the adeno-associated virus type 2 rep. In this study, a monoclonal antibody was produced against HHV-6B REP (anti-REP mAb). Immunofluorescence staining using the anti-REP mAb showed that REP was localized to the nucleus in HHV-6-infected MT4 cells. It was first detected at 24 h post-infection (p.i.) and accumulated to higher levels by 72 h p.i. REP may be expressed only at very low levels in HHV-6-infected cells: even when the late protein glycoprotein H was detected in nearly 90% of HHV-6-infected cells, REP was detected in only a small percentage of them. Western blot analysis showed that the anti-REP mAb recognized a 56-kDa polypeptide in HHV-6B-infected MT4 cells. Furthermore, the REP protein was shown to bind single-stranded DNA.
报道了人类疱疹病毒6B（HHV-6B）rep/U94基因的特征，它是2型腺相关病毒rep的同源物。在本研究中，制备了一种针对HHV-6B REP的单克隆抗体（抗REP单克隆抗体）。使用抗REP单克隆抗体进行免疫荧光染色显示，REP定位于HHV-6感染的MT4细胞的细胞核中。它在感染后24小时（p.i.）首次被检测到，并在感染后72小时积累到更高水平。REP可能在HHV-6感染的细胞中仅以非常低的水平表达：即使在近90%的HHV-6感染细胞中检测到晚期蛋白糖蛋白H时，也仅在一小部分细胞中检测到REP。蛋白质印迹分析表明，抗REP单克隆抗体在HHV-6B感染的MT4细胞中识别出一条56 kDa的多肽。此外，REP蛋白显示出与单链DNA结合。
