Caselli Elisabetta, Bracci Arianna, Galvan Monica, Boni Michela, Rotola Antonella, Bergamini Carlo, Cermelli Claudio, Dal Monte Paola, Gompels Ursula A, Cassai Enzo, Di Luca Dario
Department of Experimental and Diagnostic Medicine, Section of Microbiology, University of Ferrara, Via Borsari 46, 44100 Ferrara, Italy.
Virology. 2006 Mar 15;346(2):402-14. doi: 10.1016/j.virol.2005.11.018. Epub 2005 Dec 20.
Human herpesvirus 6 (HHV-6) is the only human herpesvirus encoding U94/rep, homologue to the parvovirus non-structural gene rep68/78. Results to date suggest that HHV-6 U94/rep might regulate viral gene expression and have a role in viral latency. To determine the effect of U94/REP upon viral replication, the protein was produced. The purified U94/REP retained the characteristic immunological features. It was internalized and localized in the nucleus of human cells, showing marked inhibitory activity on the replication of HHV-6 (both variants A and B). The effect of U94/REP was dose-dependent and sensitive to treatment with single-stranded but not double-stranded DNA. U94/REP inhibited the replication of other betaherpesviruses, HHV-7 and human cytomegalovirus, but had no effect on herpes simplex virus. These results confirm the action of U94/rep latency gene in the regulation of HHV-6 replication with implications for co-reactivations and latency of human betaherpesviruses.
人类疱疹病毒6型（HHV - 6）是唯一编码U94/rep的人类疱疹病毒，该蛋白与细小病毒的非结构基因rep68/78同源。迄今为止的研究结果表明，HHV - 6 U94/rep可能调节病毒基因表达并在病毒潜伏中发挥作用。为了确定U94/REP对病毒复制的影响，制备了该蛋白。纯化后的U94/REP保留了其特征性的免疫学特性。它被内化并定位于人类细胞的细胞核中，对HHV - 6（A和B两种变体）的复制表现出显著的抑制活性。U94/REP的作用呈剂量依赖性，并且对单链而非双链DNA处理敏感。U94/REP抑制了其他β疱疹病毒HHV - 7和人巨细胞病毒的复制，但对单纯疱疹病毒没有影响。这些结果证实了U94/rep潜伏基因在调节HHV - 6复制中的作用，这对人类β疱疹病毒的共同激活和潜伏具有重要意义。
