Ko Jong-Min, Do Geum-Sook, Suh Duck-Yong, Seo Bong-Bo, Shin Doo-Chull, Moon Huhn-Pal
National Yeongnam Agricultural Experiment Station, Milyang, Korea.
Genome. 2002 Feb;45(1):157-64. doi: 10.1139/g01-133.
Two rye genome-specific random amplified polymorphic DNA (RAPD) markers were identified for detection of rye introgression in wheat. Both markers were amplified in all of the tested materials that contained rye chromatin such as rye, hexaploid triticale, wheat-rye addition lines, and wheat varieties with 1BL.1RS translocation. Two cloned markers, designated pSc10C and pSc20H, were 1012 bp and 1494 bp, respectively. Sequence analysis showed that both pSc10C and pSc20H fragments were related to retrotransposons, ubiquitously distributed in plant genomes. Using fluorescence in situ hybridization (FISH), probe pSc10C was shown to hybridize predominantly to the pericentromeric regions of all rye chromosomes, whereas probe pSc20H was dispersed throughout the rye genome except at telomeric regions and nucleolar organizing regions. The FISH patterns showed that the two markers should be useful to select or track all wheat-rye translocation lines derived from the whole arms of rye chromosomes, as well as to characterize the positions of the translocation breakpoints generated in the proximal and distal regions of rye arms.
鉴定出两个黑麦基因组特异性随机扩增多态性DNA（RAPD）标记，用于检测小麦中的黑麦渗入。在所有含有黑麦染色质的测试材料中均扩增出这两个标记，如黑麦、六倍体小黑麦、小麦-黑麦附加系以及具有1BL.1RS易位的小麦品种。两个克隆标记，分别命名为pSc10C和pSc20H，长度分别为1012 bp和1494 bp。序列分析表明，pSc10C和pSc20H片段均与反转录转座子相关，广泛分布于植物基因组中。利用荧光原位杂交（FISH）技术，显示探针pSc10C主要与所有黑麦染色体的着丝粒区域杂交，而探针pSc20H除端粒区域和核仁组织区外，分散于整个黑麦基因组中。FISH模式表明，这两个标记对于选择或追踪源自黑麦染色体整条臂的所有小麦-黑麦易位系，以及鉴定在黑麦臂近端和远端区域产生的易位断点位置应是有用的。
