Gostimsky S A, Kokaeva Z G, Konovalov F A
Department of Genetics, Biological Faculty, Moscow State University, Moscow, 119899 Russia.
Russ J Genet. 2005;41(4):378-388. doi: 10.1007/s11177-005-0101-1.
The authors' studies on the organization and variation of plant genome with the use of molecular markers are briefly reviewed with special emphasis on random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), sequence characterized amplified region (SCAR), and cleaved amplified polymorphic sequence (CAPS) markers detected with the use of polymerase chain reaction (PCR). These markers have been demonstrated to be promising for identifying cultivars and determining the purity of genetic strains of pea. Genetic relationships between strains, cultivars, and mutants of pea have been studied. The role of molecular markers in molecular genetic mapping and localizing the genes of commercially important characters of pea has been shown. The possibility of the use of molecular markers for studying somaclonal variation and detecting mutagenic factors in plants during long-term spaceflights is considered. The prospects of using DNA markers for understanding the organization and variability of higher plant genomes are discussed.
简要回顾了作者利用分子标记对植物基因组的组织和变异进行的研究，特别强调了利用聚合酶链反应（PCR）检测的随机扩增多态性DNA（RAPD）、简单序列重复区间（ISSR）、序列特异性扩增区域（SCAR）和酶切扩增多态性序列（CAPS）标记。这些标记已被证明在鉴定豌豆品种和确定遗传菌株纯度方面很有前景。研究了豌豆菌株、品种和突变体之间的遗传关系。展示了分子标记在分子遗传图谱绘制以及定位豌豆重要商业性状基因方面的作用。考虑了利用分子标记研究体细胞克隆变异以及检测植物在长期太空飞行过程中诱变因素的可能性。讨论了使用DNA标记理解高等植物基因组组织和变异性的前景。
