Francis G W, Strand L P, Lien T, Knutsen G
Arch Microbiol. 1975 Aug 28;104(3):249-54. doi: 10.1007/BF00447333.
Synchronous cultures of Chlamydomonas reinhardii have been examined for the total amounts of carotenoid and chlorophyll present throughout a 12 hrs light -- 4 hrs dark life cycle. Variations in the carotenoid distribution at different points within the cell cycle have been found. During the greater part of the light period all major carotenoids increased at a proportionally similar rate. However, the increases in lutein and violaxanthin preceded those in beta-carotene and neoxanthin by some 2 hrs and that in loroxanthin, and algal xanthophyll, by abour 3 hrs. A marked drop in total carotenoid accumulation, corresponding to similar temporary falling away in the accumulation of beta-carotene, lutein and violaxanthin occurred at 9 hrs. The correspondence of this with the established drop in RNA accumulation and the break-up of the nucleolus was pointed out. Considerable redistribution among the carotenoids occurred during the dark period, notably the amount of beta-carotene increased relative to the total xanthophylls. The full significance of these results can not be estimated in the absence of comparative data on related organisms.
对莱茵衣藻的同步培养物进行了研究，以检测其在12小时光照 - 4小时黑暗生命周期中类胡萝卜素和叶绿素的总量。已发现细胞周期内不同时间点类胡萝卜素分布存在差异。在光照期的大部分时间里，所有主要类胡萝卜素以比例相似的速率增加。然而，叶黄素和紫黄质的增加比β-胡萝卜素和新黄质早约2小时，比洛罗黄质和藻类叶黄素早约3小时。在9小时时，类胡萝卜素总积累量显著下降，同时β-胡萝卜素、叶黄素和紫黄质的积累也出现了类似的暂时下降。指出了这与已确定的RNA积累下降和核仁解体的对应关系。在黑暗期，类胡萝卜素之间发生了相当大的重新分布，特别是β-胡萝卜素的量相对于总叶黄素增加。在缺乏相关生物体比较数据的情况下，无法评估这些结果的全部意义。
