Sibille I, Duverneuil C, Lorin de la Grandmaison G, Guerrouache K, Teissière F, Durigon M, de Mazancourt P
Department of Pathology and Forensic Medicine, Raymond Poincaré Hospital, 92380 Garches, France.
Forensic Sci Int. 2002 Feb 18;125(2-3):212-6. doi: 10.1016/s0379-0738(01)00650-8.
Identification of spermatozoa is the biological evidence most often sought in specimens from rape victims. Absence of spermatozoa usually terminates biological investigations, and the victim's testimony can be contested. We assessed the utility and reliability of PCR amplification using Y-chromosomal STR polymorphisms in specimens from female victims of sexual assault with negative cytology. One hundred and four swabs without spermatozoa detected by cytology were collected from 79 alleged sexually assaulted female victims and amplification of Y-STR and of amelogenin was performed.Overall, Y-chromosome was detected and evidenced sexual penetration in 28.8% of swabs. In the population of victims examined more than 48 h after the sexual assault, Y-STR were still evidenced in 30% of the cases. These results show that swabs should be taken from victims for Y-chromosome DNA typing even after long delays between sexual assault and medical examination.
精子鉴定是强奸受害者样本中最常寻找的生物学证据。未发现精子通常会终止生物学调查，受害者的证词可能会受到质疑。我们评估了在细胞学检查为阴性的性侵犯女性受害者样本中，使用Y染色体短串联重复序列（STR）多态性进行PCR扩增的实用性和可靠性。从79名声称遭受性侵犯的女性受害者中收集了104份经细胞学检查未发现精子的拭子，并进行了Y-STR和牙釉蛋白的扩增。总体而言，在28.8%的拭子中检测到Y染色体并证明有性侵犯。在性侵犯发生48小时后接受检查的受害者群体中，30%的病例仍能检测到Y-STR。这些结果表明，即使在性侵犯和医学检查之间间隔很长时间后，也应从受害者身上采集拭子进行Y染色体DNA分型。
