Myskowiak Jean Bernard, Doums Claudie
Institut de Recherche Criminelle de la Gendarmerie Nationale, 1 Bd Théophile Sueur, 93111 Rosny-sous-Bois Cedex, France.
Forensic Sci Int. 2002 Feb 18;125(2-3):254-61. doi: 10.1016/s0379-0738(02)00003-8.
The aim of this study was to simulate the low temperatures that insects could experience between the time being sampled from cadavers and their arrival in the laboratory. This was in order to investigate the effect of low temperature on development of maggots. At different stages of development, individuals of Protophormia terraenovae (Robineau-Desvoidy) reared at 24 degrees C were submitted to a temperature of 4.0+/-0.5 degrees C for a period varying from 1 to 10 days. Independent of the stage of development at which the insects were refrigerated, the treatment induced significant changes on the duration of development. The effect of low temperature on the developmental time between the return to 24 degrees C to adult emergence depended on the larval stage that was refrigerated. When first instar larvae and prepupae were refrigerated, the time to emergence at 24 degrees C decreased with an increase of duration of the refrigeration period. Time to emergence increased under the same conditions when second instar larvae and pupae were refrigerated. These results indicate that keeping larvae of P. terraenovae at 4 degrees C does not just simply lead to a cessation of metabolism but disturbs the regular development. Ten days of cooling induced an error in estimating post-mortem interval (PMI) of more than 6h.
本研究的目的是模拟昆虫从尸体上被采集到抵达实验室这段时间可能经历的低温。这样做是为了研究低温对蛆发育的影响。在不同发育阶段，将在24摄氏度饲养的新陆原伏蝇（Robineau-Desvoidy）个体置于4.0±0.5摄氏度的温度下1至10天。无论昆虫在哪个发育阶段被冷藏，这种处理都会对发育持续时间产生显著影响。从恢复到24摄氏度到成虫羽化之间的发育时间受低温的影响取决于被冷藏的幼虫阶段。当一龄幼虫和预蛹被冷藏时，在24摄氏度下的羽化时间会随着冷藏期持续时间的增加而减少。当二龄幼虫和蛹被冷藏时，在相同条件下羽化时间会增加。这些结果表明，将新陆原伏蝇幼虫置于4摄氏度下不仅会简单地导致新陈代谢停止，还会干扰正常发育。十天的冷藏会导致死后间隔时间（PMI）估计出现超过6小时的误差。
