Hoh Christoph, Dudziak Gregor, Liese Andreas
Forschungszentrum Jülich GmbH, Institut für Biotechnologie 2, D-52425 Jülich, Germany.
Bioorg Med Chem Lett. 2002 Apr 8;12(7):1031-4. doi: 10.1016/s0960-894x(02)00087-2.
The enzymatic synthesis of Gal-beta 1,3[GlcNAc-beta 1,6]-GalNAc-alpha 1-OBn (core 2-Bn) using a multi-enzyme system consisting of a beta-galactosidase (EC 3.2.1.23) from bovine testes and a recombinant core 2 beta 1,6-GlcNAc transferase (C2GnT, EC 2.4.1.102) was empirically optimized by the use of a genetic algorithm. After variation of seven relevant parameters and performance of 56 experiments, two local maxima regarding the selection criteria could be found after four generations of optimization. The selectivity of core 2-Bn formation showed values up to 90%.
使用由牛睾丸中的β-半乳糖苷酶（EC 3.2.1.23）和重组核心2β1,6-N-乙酰葡糖胺转移酶（C2GnT，EC 2.4.1.102）组成的多酶系统，通过遗传算法对Gal-β1,3[GlcNAc-β1,6]-GalNAc-α1-OBn（核心2-Bn）的酶促合成进行了经验优化。在七个相关参数变化并进行了56次实验后，经过四代优化，发现了关于选择标准的两个局部最大值。核心2-Bn形成的选择性高达90%。
