In situ generated O-glycan core 1 structure as substrate for Gal(beta 1-3)GalNAc beta-1,6-GlcNAc transferase.

作者信息

Dudziak G, Zeng S, Berger E G, Gallego R G, Kamerling J P, Kragl U, Wandrey C

机构信息

Forschungszentrum Jülich GmbH, Institut für Biotechnologie, Jülich, Germany.

出版信息

Bioorg Med Chem Lett. 1998 Sep 22;8(18):2595-8. doi: 10.1016/s0960-894x(98)00464-8.

beta-Galactosidase from bovine testes was used in a one pot reaction together with a recombinant beta-1,6-GlcNAc transferase for the synthesis of GlcNAc(beta 1-6)GalNAc(alpha 1-OBn) (core 6-Bn). The galactosidase, which reversibly links galactose via a (beta 1-3) linkage to N-acetylgalactosamine, provides the substrate for the GlcNAc transferase in situ. The synthesis was carried out with a yield > 90%.