Villaseñor Irene M, Echegoyen Deborah E, Angelada Jennifer S
Institute of Chemistry and Natural Sciences Research Institute, University of the Philippines, Diliman, 1101, Quezon City, Philippines.
Mutat Res. 2002 Mar 25;515(1-2):141-6. doi: 10.1016/s1383-5718(02)00006-2.
The CHCl(3) extract of Mentha cordifolia Opiz. showed antimutagenicity against tetracycline. An antimutagen was purified by solvent partitioning and repeated normal phase-vacuum liquid chromatography (NP-VLC) using a micronucleus test-guided isolation and purification. Spectral analyses showed that the isolated antimutagen is possibly 6,7-bis-(2,2-dimethoxyethene)-2,11-dimethoxy-2Z,4E,8E,10Z-dodecatetraendioic acid. It inhibited the mutagenicity of tetracycline by 68.7% at a dosage of 0.01 mg per 20 g mouse. Statistical analysis using Kruskal-Wallis one-way analysis of variance (ANOVA) by ranks showed that its variance differs from that of the solvent control group (tetracycline+dimethylsulfoxide (DMSO)) at alpha=0.001. Moreover, the isolated antimutagen did not exhibit mutagenic activity at the same dosage. Statistical analysis showed that it is not mutagenic at 0.001 level of significance because its variance differs from that of tetracycline.
薄荷（Mentha cordifolia Opiz.）的氯仿提取物对四环素显示出抗诱变活性。通过溶剂分配和重复的正相真空液相色谱法（NP-VLC），利用微核试验指导的分离和纯化方法对一种抗诱变剂进行了纯化。光谱分析表明，分离得到的抗诱变剂可能是6,7-双-（2,2-二甲氧基乙烯）-2,11-二甲氧基-2Z,4E,8E,10Z-十二碳四烯二酸。在每20克小鼠0.01毫克的剂量下，它对四环素诱变活性的抑制率为68.7%。使用Kruskal-Wallis秩和单向方差分析（ANOVA）进行的统计分析表明，在α=0.001时，其方差与溶剂对照组（四环素+二甲基亚砜（DMSO））不同。此外，分离得到的抗诱变剂在相同剂量下未表现出诱变活性。统计分析表明，在0.001的显著性水平下它不具有诱变作用，因为其方差与四环素的方差不同。
