Villaseñor I M, Edu D A
Institute of Chemistry, University of the Philippines, Dilman, Quezon City.
Mutat Res. 1993 Jan;298(3):215-8. doi: 10.1016/0165-1218(93)90043-d.
An antimutagenic principle was extracted from the leaves of Carmona retusa with ethyl alcohol. This was then purified by solvent partition and liquid chromatography. The micronucleus test, an in vivo method, using albino mice as the test system was used for monitoring the antimutagenic activity. At a dosage of 23.4 mg/kg body weight, the pure isolate reduced by 68.4% the number of micronucleated polychromatic erythrocytes induced by the mutagen tetracycline. Statistical analysis (one-way ANOVA) shows that the variance of the pure isolate differs significantly from that of tetracycline, a known mutagen.
用乙醇从基及树的叶子中提取了一种抗诱变成分。然后通过溶剂分配和液相色谱法对其进行纯化。采用微核试验这一体内方法，以白化小鼠作为试验系统来监测抗诱变活性。在体重剂量为23.4 mg/kg时，纯分离物使诱变剂四环素诱导产生的微核多染红细胞数量减少了68.4%。统计分析（单向方差分析）表明，纯分离物的方差与已知诱变剂四环素的方差有显著差异。
