典型异尖线虫（Diesing，1860）研究中的遗传标记：幼虫鉴定及其与其他异尖线虫属物种（Dujardin，1845）（线虫纲：异尖科）的遗传关系
Genetic markers in the study of Anisakis typica (Diesing, 1860): larval identification and genetic relationships with other species of Anisakis Dujardin, 1845 (Nematoda: Anisakidae).
作者信息
Mattiucci S, Paggi L, Nascetti G, Portes Santos C, Costa G, Di Beneditto A P, Ramos R, Argyrou M, Cianchi R, Bullini L
机构信息
Department of Public Health Science, Section of Parasitology, University of Rome 'La Sapienza', P. le Aldo Moro, 5, I-00185 Rome, Italy.
出版信息
Syst Parasitol. 2002 Mar;51(3):159-70. doi: 10.1023/a:1014554900808.
Genetic variation at 21 gene-enzyme systems was studied in a sample of an adult population of Anisakis typica (Diesing, 1860) recovered in the dolphin Sotalia fluviatilis from the Atlantic coast of Brazil. The characteristic alleles, detected in this population, made it possible to identify as A. typica, Anisakis larvae with a Type I morphology (sensu Berland, 1961) from various fishes: Thunnus thynnus and Auxis thazard from Brazil waters, Trachurus picturatus and Scomber japonicus from Madeiran waters, Scomberomorus commerson, Euthynnus affinis, Sarda orientalis and Coryphaena hippurus from the Somali coast of the Indian Ocean, and Merluccius merluccius from the Eastern Mediterranean. Characteristic allozymes are given for the identification, at any life-stage and in both sexes, of A. typica and the other Anisakis species so far studied genetically. The distribution of A. typica in warmer temperate and tropical waters is confirmed; the definitive hosts so far identified for this species belong to delphinids, phocoenids and pontoporids. The present findings represent the first established records of intermediate/paratenic hosts of A. typica and extend its range to Somali waters of the Indian Ocean and to the Eastern Mediterranean Sea. A remarkable genetic homogeneity was observed in larval and adult samples of A. typica despite their different geographical origin; interpopulation genetic distances were low, ranging from D(Nei)=0.004 (Eastern Mediterranean versus Somali) to D(Nei)=0.010 (Brazilian versus Somali). Accordingly, indirect estimates of gene flow gave a rather high average value of Nm = 6.00. Genetic divergence of A. typica was, on average, D(Nei)=1.12 from the members of the A. simplex complex (A. simplex s.s, A. pegreffii, A. simplex C) and D(Nei)=1.41 from A. ziphidarum, which all share Type I larvae; higher values were found from both A. physeteris (D(Nei)=2.77)
在从巴西大西洋沿岸的淡水海豚（Sotalia fluviatilis）体内采集的典型异尖线虫（Anisakis typica，Diesing，1860）成虫样本中，研究了21个基因 - 酶系统的遗传变异。在该种群中检测到的特征等位基因，使得能够将来自各种鱼类的具有I型形态（根据Berland，1961）的异尖线虫幼虫鉴定为典型异尖线虫：来自巴西海域的蓝鳍金枪鱼（Thunnus thynnus）和扁舵鲣（Auxis thazard），来自马德拉群岛海域的高背鲭（Trachurus picturatus）和日本鲭（Scomber japonicus），来自印度洋索马里海岸的康氏马鲛（Scomberomorus commerson）、黄鳍金枪鱼（Euthynnus affinis）、东方狐鲣（Sarda orientalis）和普通鲯鳅（Coryphaena hippurus），以及来自东地中海的无须鳕（Merluccius merluccius）。给出了特征性同工酶，用于在任何生命阶段和两性中鉴定典型异尖线虫以及迄今为止进行过遗传研究的其他异尖线虫物种。典型异尖线虫在温暖的温带和热带水域中的分布得到证实；迄今为止确定的该物种的终末宿主属于海豚科、鼠海豚科和拉河豚科。目前的研究结果代表了典型异尖线虫中间/转续宿主的首次既定记录，并将其分布范围扩展到印度洋的索马里海域和东地中海。尽管典型异尖线虫的幼虫和成虫样本地理来源不同，但观察到显著的遗传同质性；种群间遗传距离较低，范围从D（Nei）=0.004（东地中海与索马里）到D（Nei）=0.010（巴西与索马里）。因此，基因流的间接估计得出相当高的平均Nm值 = 6.00。典型异尖线虫与简单异尖线虫复合体（简单异尖线虫指名亚种、佩氏异尖线虫、简单异尖线虫C）成员的平均遗传分歧为D（Nei）=1.12，与Ziphiidarum异尖线虫的平均遗传分歧为D（Nei）=1.41，它们都具有I型幼虫；与Physeteris异尖线虫的遗传分歧更高（D（Nei）=2.77）
Zotero 插件