Hecht N B
Biochim Biophys Acta. 1975 Nov 18;414(1):59-70. doi: 10.1016/0005-2787(75)90125-2.
A DNA-directed DNA polymerase has been isolated from murine liver mitochondria. The mitochondrial DNA polymerase is distinguishable from other DNA polymerases found in the nucleus and cytosol of murine cells by several enzymatic and physical properties. It is stimulated 5--6-fold by 0.15 M KCl, does not require a sulfhydryl reducing agent for activity, and is inhibited by ethidium bromide or ATP. The enzyme has a sedimentation coefficient of 8.8 S in the presence of up to 0.5 M KCl, a molecular weight of 150--170000, and utilizes natural templates in the following order of preference: activated DNA (100%), single stranded DNA (24%), and native DNA (5%).
已从小鼠肝脏线粒体中分离出一种DNA指导的DNA聚合酶。该线粒体DNA聚合酶在若干酶学和物理特性方面有别于在小鼠细胞核和细胞质中发现的其他DNA聚合酶。它在0.15M KCl存在下活性可被刺激5至6倍，其活性不需要巯基还原剂，并且会被溴化乙锭或ATP抑制。在存在高达0.5M KCl的情况下，该酶的沉降系数为8.8S，分子量为150至170000，并且按以下优先顺序利用天然模板：活化DNA（100%）、单链DNA（24%）和天然DNA（5%）。
